PUBLICATION
The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males
- Authors
- Zucchi, S., Blüthgen, N., Ieronimo, A., and Fent, K.
- ID
- ZDB-PUB-101018-21
- Date
- 2011
- Source
- Toxicology and applied pharmacology 250(2): 137-146 (Journal)
- Registered Authors
- Keywords
- UV-filter, UV-absorber, Benzophenone-4, Effects on eleuthero embryos, mRNA expression, Transcription profile
- MeSH Terms
-
- Animals
- Benzophenones/administration & dosage
- Benzophenones/toxicity*
- Brain/drug effects
- Brain/metabolism
- Dose-Response Relationship, Drug
- Estrogen Antagonists/pharmacology
- Estrogens/pharmacology
- Gene Expression Regulation/drug effects*
- Gene Expression Regulation, Developmental/drug effects*
- Liver/drug effects
- Liver/metabolism
- Male
- Sunscreening Agents/administration & dosage
- Sunscreening Agents/toxicity*
- Testis/drug effects
- Testis/metabolism
- Thyroid Gland/drug effects
- Thyroid Gland/embryology
- Transcription, Genetic/drug effects
- Zebrafish
- PubMed
- 20937294 Full text @ Tox. App. Pharmacol.
- CTD
- 20937294
Citation
Zucchi, S., Blüthgen, N., Ieronimo, A., and Fent, K. (2011) The UV-absorber benzophenone-4 alters transcripts of genes involved in hormonal pathways in zebrafish (Danio rerio) eleuthero-embryos and adult males. Toxicology and applied pharmacology. 250(2):137-146.
Abstract
Benzophenone-4 (BP-4) is frequently used as UV-absorber in cosmetics and materials protection. Despite its frequent detection in the aquatic environment potential effects on aquatic life are unknown. In this study, we evaluate the effects of BP-4 in eleuthero-embryos and in the liver, testis and brain of adult male fish on the transcriptional level by focusing on target genes involved in hormonal pathways to provide a more complete toxicological profile of this important UV-absorber. Eleuthero-embryos and males of zebrafish were exposed up to 3days after hatching and for 14days, respectively, to BP-4 concentrations between 30 and 3000μg/L. In eleuthero-embryos transcripts of vtg1, vtg3, esr1, esr2b, hsd17β3, cyp19b cyp19a, hhex and pax8 were induced at 3000μg/L BP-4, which points to a low estrogenic activity and interference with early thyroid development, respectively. In adult males BP-4 displayed multiple effects on gene expression in different tissues. In the liver vtg1, vtg3, esr1 and esr2b were down-regulated, while in the brain, vtg1, vtg3 and cyp19b transcripts were up-regulated. In conclusion, the transcription profile revealed that BP-4 interferes with the expression of genes involved in hormonal pathways and steroidogenesis. The effects of BP-4 differ in life stages and adult tissues and point to an estrogenic activity in eleuthero-embryos and adult brain, and an antiestrogenic activity in the liver. The results indicate that BP-4 interferes with the sex hormone system of fish, which is important for the risk assessment of this UV-absorber.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping