PUBLICATION
Dynamic transcriptomic profiles of zebrafish gills in response to zinc supplementation
- Authors
- Zheng, D., Kille, P., Feeney, G.P., Cunningham, P., Handy, R.D., and Hogstrand, C.
- ID
- ZDB-PUB-101018-20
- Date
- 2010
- Source
- BMC Genomics 11: 553 (Journal)
- Registered Authors
- Keywords
- none
- Datasets
- GEO:GSE21914, GEO:GSE21907, GEO:GSE21894
- MeSH Terms
-
- 5' Untranslated Regions/genetics
- Animals
- Binding Sites
- Dietary Supplements*
- Gene Expression Profiling*
- Gene Expression Regulation/drug effects
- Gene Regulatory Networks/genetics
- Gills/drug effects*
- Gills/metabolism*
- Molecular Sequence Annotation
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Reproducibility of Results
- Reverse Transcriptase Polymerase Chain Reaction
- Signal Transduction/drug effects
- Signal Transduction/genetics
- Time Factors
- Transcription Factors/metabolism
- Transcription, Genetic/drug effects
- Zebrafish/genetics*
- Zebrafish/physiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Zinc/pharmacology*
- PubMed
- 20937081 Full text @ BMC Genomics
Citation
Zheng, D., Kille, P., Feeney, G.P., Cunningham, P., Handy, R.D., and Hogstrand, C. (2010) Dynamic transcriptomic profiles of zebrafish gills in response to zinc supplementation. BMC Genomics. 11:553.
Abstract
BACKGROUND: Dietary zinc supplementation may help to promote growth, boost the immune system, protect against diabetes, and aid recovery from diarrhoea. We exploited the zebrafish (Danio rerio) gill as a unique vertebrate ion transporting epithelium model to study the time-dependent regulatory networks of gene-expression leading to homeostatic control during zinc supplementation. This organ forms a conduit for zinc uptake whilst exhibiting conservation of zinc trafficking components.
RESULTS: Fish were maintained with either zinc supplemented water (4.0 M) and diet (2023 mg zinc kg-1) or water and diet containing Zn2+ at 0.25 uM and 233 mg zinc kg-1, respectively. Gill tissues were harvested at five time points (8 hours to 14 days) and transcriptome changes analysed in quintuplicate using a 16K microarray with results anchored to gill Zn2+ influx and whole body nutrient composition (protein, carbohydrate, lipid, elements). The number of regulated genes increased up to day 7 but declined as the fish acclimated. In total 525 genes were regulated (having a fold-change more than 1.8 fold change and an adjusted P-value less than 0.1 which is controlling a 10% False discovery rate, FDR) by zinc supplementation, but little overlap was observed between genes regulated at successive time-points. Many genes displayed cyclic expression, typical for homeostatic control mechanisms. Annotation enrichment analysis revealed strong overrepresentation of "transcription factors", with specific association evident with "steroid hormone receptors". A suite of genes linked to "development" were also statistically overrepresented. More specifically, early regulation of genes was linked to a few key transcription factors (e.g. Mtf1, Jun, Stat1, Ppara, Gata3) and was followed by hedgehog and bone morphogenic protein signalling.
CONCLUSIONS: The results suggest that zinc supplementation reactivated developmental pathways in the gill and stimulated stem cell differentiation, a response likely reflecting gill remodelling in response to its altered environment. This provides insight to the role of zinc during cell differentiation and illustrates the critical nature of maintaining zinc status. The study also highlights the importance of temporal transcriptomics analysis in order resolve the discrete elements of biological processes, such as zinc acclimation.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping