PUBLICATION
Effects of Lactobacillus rhamnosus on zebrafish oocyte maturation: an FTIR imaging and biochemical analysis
- Authors
- Giorgini, E., Conti, C., Ferraris, P., Sabbatini, S., Tosi, G., Rubini, C., Vaccari, L., Gioacchini, G., and Carnevali, O.
- ID
- ZDB-PUB-101018-17
- Date
- 2010
- Source
- Analytical and bioanalytical chemistry 398(7-8): 3063-3072 (Journal)
- Registered Authors
- Carnevali, Oliana
- Keywords
- FPA FTIR imaging, Vibrational analysis, Fish oocytes, Probiotic, Ovary, Danio rerio
- MeSH Terms
-
- Animals
- Cathepsin L/genetics
- Cathepsin L/physiology
- Electrophoresis, Polyacrylamide Gel
- Female
- Lacticaseibacillus rhamnosus/physiology*
- Oocytes/enzymology
- Oocytes/microbiology*
- Oocytes/physiology
- Probiotics/pharmacology*
- RNA/chemistry
- RNA/genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Spectroscopy, Fourier Transform Infrared/methods*
- Zebrafish
- PubMed
- 20936269 Full text @ Anal. Bioanal. Chem.
Citation
Giorgini, E., Conti, C., Ferraris, P., Sabbatini, S., Tosi, G., Rubini, C., Vaccari, L., Gioacchini, G., and Carnevali, O. (2010) Effects of Lactobacillus rhamnosus on zebrafish oocyte maturation: an FTIR imaging and biochemical analysis. Analytical and bioanalytical chemistry. 398(7-8):3063-3072.
Abstract
The aim of this study was to verify the effects of probiotic Lactobacillus rhamnosus on zebrafish oocyte maturation using FPA (focal plane array) FTIR imaging together with specific biochemical assays (SDS-PAGE, real-time PCR and enzymatic assay). Oocyte growth is prevalently due to a vitellogenic process which consists of the hepatic synthesis of vitellogenin and its selective uptake during maturation. The administration of L. rhamnosus IMC 501 for 10 days induced chemical changes to oocyte composition, promoting the maturation process. Some interesting biochemical features, linked to protein secondary structure (amide I band) and to phospholipidic and glucidic patterns, were detailed by vibrational analysis. The spectroscopic results were supported by the early increase of the lysosomal enzyme involved in the final oocyte maturation, the cathepsin L. This enzyme increases during follicle maturation, with the highest levels in class IV oocytes. In treated females, class III oocytes showed higher cathepsin L gene expression and enzymatic activity, with levels comparable to class IV oocytes isolated from controls; this can be related to the proteolytic cleavage of the higher molecular mass yolk protein components, as evidenced by SDS-PAGE.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping