PUBLICATION
Circadian and homeostatic regulation of structural synaptic plasticity in hypocretin neurons
- Authors
- Appelbaum, L., Wang, G., Yokogawa, T., Skariah, G.M., Smith, S.J., Mourrain, P., and Mignot, E.
- ID
- ZDB-PUB-101011-46
- Date
- 2010
- Source
- Neuron 68(1): 89-98 (Journal)
- Registered Authors
- Appelbaum, Lior, Mourrain, Philippe
- Keywords
- none
- MeSH Terms
-
- Animals
- Synaptophysin/metabolism
- Animals, Genetically Modified
- Light
- Neurons/cytology
- Neurons/physiology*
- Melatonin/pharmacology
- C-Reactive Protein/genetics
- C-Reactive Protein/metabolism
- Neuronal Plasticity/genetics
- Neuronal Plasticity/physiology*
- Green Fluorescent Proteins/genetics
- Larva
- In Vitro Techniques
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Homeostasis/genetics
- Homeostasis/physiology*
- Orexins
- Neuropeptides/metabolism*
- Humans
- Synapses/genetics
- Synapses/physiology*
- Pineal Gland/growth & development
- Pineal Gland/metabolism
- Intracellular Signaling Peptides and Proteins/metabolism*
- Axons/metabolism
- Behavior, Animal
- Circadian Rhythm/genetics
- Circadian Rhythm/physiology*
- Microscopy, Confocal
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Brain/cytology
- Brain/growth & development
- Zebrafish
- PubMed
- 20920793 Full text @ Neuron
Citation
Appelbaum, L., Wang, G., Yokogawa, T., Skariah, G.M., Smith, S.J., Mourrain, P., and Mignot, E. (2010) Circadian and homeostatic regulation of structural synaptic plasticity in hypocretin neurons. Neuron. 68(1):89-98.
Abstract
Neurons exhibit rhythmic activity that ultimately affects behavior such as sleep. In living zebrafish larvae, we used time-lapse two-photon imaging of the presynaptic marker synaptophysin in hypocretin/orexin (HCRT) neurons to determine the dynamics of synaptic modifications during the day and night. We observed circadian rhythmicity in synapse number in HCRT axons. This rhythm is regulated primarily by the circadian clock but is also affected by sleep deprivation. Furthermore, NPTX2, a protein implicated in AMPA receptor clustering, modulates circadian synaptic changes. In zebrafish, nptx2b is a rhythmic gene that is mostly expressed in hypothalamic and pineal gland cells. Arrhythmic transgenic nptx2b overexpression (hcrt:NPTX2b) increases synapse number and abolishes rhythmicity in HCRT axons. Finally, hcrt:NPTX2b fish are resistant to the sleep-promoting effects of melatonin. This behavioral effect is consistent with NPTX2b-mediated increased activity of HCRT circuitry. These data provide real-time in vivo evidence of circadian and homeostatic regulation of structural synaptic plasticity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping