PUBLICATION

Schwann cells reposition a peripheral nerve to isolate it from postembryonic remodeling of its targets

Authors
Raphael, A.R., Perlin, J.R., and Talbot, W.S.
ID
ZDB-PUB-101011-16
Date
2010
Source
Development (Cambridge, England)   137(21): 3643-3649 (Journal)
Registered Authors
Perlin, Julie, Raphael, Alya, Talbot, William S.
Keywords
none
MeSH Terms
  • Animals
  • Cell Movement/physiology*
  • Epidermis/growth & development*
  • Epidermis/innervation*
  • Models, Biological
  • Animals, Genetically Modified
  • Embryo, Nonmammalian
  • Zebrafish/embryology
  • Zebrafish/physiology
  • Peripheral Nerves/physiology*
  • Basement Membrane/innervation
  • Basement Membrane/physiology
  • Nerve Regeneration/physiology
  • Schwann Cells/physiology*
(all 14)
PubMed
20876648 Full text @ Development
Abstract
Although much is known about the initial construction of the peripheral nervous system (PNS), less well understood are the processes that maintain the position and connections of nerves during postembryonic growth. Here, we show that the posterior lateral line nerve in zebrafish initially grows in the epidermis and then rapidly transitions across the epidermal basement membrane into the subepidermal space. Our experiments indicate that Schwann cells, which myelinate axons in the PNS, are required to reposition the nerve. In mutants lacking Schwann cells, the nerve is mislocalized and the axons remain in the epidermis. Transplanting wild-type Schwann cells into these mutants rescues the position of the nerve. Analysis of chimeric embryos suggests that the process of nerve relocalization involves two discrete steps - the degradation and recreation of the epidermal basement membrane. Although the outgrowth of axons is normal in mutants lacking Schwann cells, the nerve becomes severely disorganized at later stages. In wild-type embryos, exclusion of the nerve from the epidermis isolates axons from migration of their targets (sensory neuromasts) within the epidermis. Without Schwann cells, axons remain within the epidermis and are dragged along with the migrating neuromasts. Our analysis of the posterior lateral line system defines a new process in which Schwann cells relocate a nerve beneath the epidermal basement membrane to insulate axons from the postembryonic remodeling of their targets.
Genes / Markers
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Expression
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Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
st48
    		Point Mutation
    st61
      		Point Mutation
      t3
        		Insertion
        zf15TgTransgenic Insertion
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          Human Disease / Model
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          Sequence Targeting Reagents
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          Fish
          Fish
          erbb2st61/st61
          erbb3bst48/st48
          sox10t3/t3
          zf15Tg
          1 - 4 of 4
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          Orthology
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          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          1 - 1 of 1
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          Mapping
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          Citation
          Raphael, A.R., Perlin, J.R., and Talbot, W.S. (2010) Schwann cells reposition a peripheral nerve to isolate it from postembryonic remodeling of its targets. Development (Cambridge, England). 137(21):3643-3649.