PUBLICATION

Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach

Authors
Encinas, P., Rodriguez-Milla, M.A., Novoa, B., Estepa, A., Figueras, A., and Coll, J.
ID
ZDB-PUB-101011-12
Date
2010
Source
BMC Genomics   11: 518 (Journal)
Registered Authors
Figueras, Antonio, Novoa, Beatriz
Keywords
none
Datasets
GEO:GSE19049, GEO:GSE19503
MeSH Terms
  • Gene Expression Profiling*
  • Polymerase Chain Reaction
  • Electrophoresis, Gel, Two-Dimensional
  • Rhabdoviridae/physiology*
  • Time Factors
  • Zebrafish/genetics
  • Zebrafish/immunology*
  • Zebrafish/virology
  • Acclimatization/genetics
  • Acclimatization/immunology
  • Hemorrhagic Septicemia, Viral/genetics*
  • Hemorrhagic Septicemia, Viral/immunology
  • Hemorrhagic Septicemia, Viral/mortality*
  • Hemorrhagic Septicemia, Viral/virology
  • Gene Expression Regulation
  • Organ Specificity/genetics
  • Proteomics*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Animals
  • Oligonucleotide Array Sequence Analysis
  • Fish Proteins/genetics
  • Fish Proteins/metabolism
  • Animal Fins/immunology*
  • Animal Fins/metabolism
  • Animal Fins/virology
(all 26)
PubMed
20875106 Full text @ BMC Genomics
Abstract
BACKGROUND: Despite rhabdoviral infections being one of the best known fish diseases, the gene expression changes induced at the surface tissues after the natural route of infection (infection-by-immersion) have not been described yet. This work describes the differential infected versus non-infected expression of proteins and immune-related transcripts in fins and organs of zebrafish Danio rerio shortly after infection-by-immersion with viral haemorrhagic septicemia virus (VHSV). RESULTS: Two-dimensional differential gel electrophoresis detected variations on the protein levels of the enzymes of the glycolytic pathway and cytoskeleton components but it detected very few immune-related proteins. Differential expression of immune-related gene transcripts estimated by quantitative polymerase chain reaction arrays and hybridization to oligo microarrays showed that while more transcripts increased in fins than in organs (spleen, head kidney and liver), more transcripts decreased in organs than in fins. Increased differential transcript levels in fins detected by both arrays corresponded to previously described infection-related genes such as complement components (c3b, c8 and c9) or class I histocompatibility antigens (mhc1) and to newly described genes such as secreted immunoglobulin domain (sid4), macrophage stimulating factor (mst1) and a cluster differentiation antigen (cd36). CONCLUSIONS: The genes described would contribute to the knowledge of the earliest molecular events occurring in the fish surfaces at the beginning of natural rhabdoviral infections and/or might be new candidates to be tested as adjuvants for fish vaccines.
Genes / Markers
Figures
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
No data available
Fish
No data available
Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Encinas, P., Rodriguez-Milla, M.A., Novoa, B., Estepa, A., Figueras, A., and Coll, J. (2010) Zebrafish fin immune responses during high mortality infections with viral haemorrhagic septicemia rhabdovirus. A proteomic and transcriptomic approach. BMC Genomics. 11:518.