PUBLICATION
A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae
- Authors
- Wan, G., and Chan, K.M.
- ID
- ZDB-PUB-100910-4
- Date
- 2010
- Source
- Journal of molecular endocrinology 45(5): 301-315 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Actins/genetics
- Actins/metabolism
- Animals
- Animals, Genetically Modified
- Blotting, Southern
- Catalase/metabolism
- Cloning, Molecular
- Fatty Acid Synthases/chemistry
- Fatty Acid Synthases/genetics
- Fatty Acid Synthases/metabolism
- Fish Proteins/genetics*
- Fish Proteins/metabolism*
- Gene Expression
- Glutathione Transferase/genetics
- Glutathione Transferase/metabolism
- Glycoproteins/genetics*
- Glycoproteins/metabolism*
- Green Fluorescent Proteins/genetics
- Insulin/genetics
- Insulin/metabolism
- Larva/genetics
- Larva/metabolism
- Leptin/genetics
- Leptin/metabolism
- Pituitary Hormones/genetics*
- Pituitary Hormones/metabolism*
- Pro-Opiomelanocortin/genetics
- Pro-Opiomelanocortin/metabolism
- Somatomedins/genetics
- Somatomedins/metabolism
- Sterol Regulatory Element Binding Protein 1/genetics
- Sterol Regulatory Element Binding Protein 1/metabolism
- Vitellogenins/chemistry
- Vitellogenins/genetics
- Vitellogenins/metabolism
- Zebrafish*/genetics
- Zebrafish*/growth & development
- Zebrafish*/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 20801895 Full text @ J. Mol. Endocrinol.
Citation
Wan, G., and Chan, K.M. (2010) A study of somatolactin actions by ectopic expression in transgenic zebrafish larvae. Journal of molecular endocrinology. 45(5):301-315.
Abstract
Somatolactin (SL) is a fish-specific hormone that belongs to the prolactin (PRL) and growth hormone (GH) family. Recently, two forms of SL, SLalpha and SLbeta, have been found in some species, and may have different actions and functions. To investigate the role of SL in fish growth and metabolism, we generated transgenic fish founders with ectopic expression of SLalpha and SLbeta to study the physiological functions and actions of these SLs among several marker genes. We fused the cDNAs encoding the precursor SLs in frame to a zebrafish beta-actin gene promoter to generate transgenic zebrafish lines that were coinjected with a GFP protein driven by the same promoter. The transgenic zebrafish were selected based on GFP expression and confirmed by genomic polymerase chain reaction (PCR), Southern blot analysis, and transgene expression. Investigations into the expression of marker genes in larvae on different pathways using real-time PCR have provided a general understanding of the actions of SLs. This study found that the overexpression of SLalpha and SLbeta in vivo significantly enhanced the transcription of insulin-like growth factors (IGFs), insulin, leptin, sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FAS), as well as the expression level of vitellogenin (VTG) and melanocyte-stimulating hormone (MSH), while causing reduced levels of catalase (CAT) and glutathione S-transferase (GST) in the larvae of transgenic zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping