PUBLICATION
Mtmr8 is essential for vasculature development in zebrafish embryos
- Authors
- Mei, J., Liu, S., Li, Z., and Gui, J.F.
- ID
- ZDB-PUB-100910-31
- Date
- 2010
- Source
- BMC Developmental Biology 10: 96 (Journal)
- Registered Authors
- Li, Zhi, Mei, Jie
- Keywords
- none
- MeSH Terms
-
- Animals
- Blood Vessels/embryology*
- Embryo, Nonmammalian/metabolism
- Hedgehog Proteins/metabolism*
- Organogenesis*
- Phosphatidylinositol 3-Kinases/metabolism
- Protein Tyrosine Phosphatases, Non-Receptor/metabolism*
- Signal Transduction
- Vascular Endothelial Growth Factor A/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism*
- PubMed
- 20815916 Full text @ BMC Dev. Biol.
Citation
Mei, J., Liu, S., Li, Z., and Gui, J.F. (2010) Mtmr8 is essential for vasculature development in zebrafish embryos. BMC Developmental Biology. 10:96.
Abstract
BACKGROUND: Embryonic morphogenesis of vascular and muscular systems is tightly coordinated, and a functional cooperation of Mtmr8 with PI3K in actin filament modeling and muscle development has been revealed in zebrafish. Here, we attempt to explore the function of Mtmr8 in vasculature development parallel to its function in muscle development.
RESULTS: During early stage of somitogenesis, mtmr8 expression was detected in both somitic mesodem and ventral mesoderm. Knockdown of mtmr8 by morpholino impairs arterial endothelial marker expression, and results in endothelial cell reduction and vasculogenesis defects, such as retardation in intersegmental vessel development and interruption of trunk dorsal aorta. Moreover, mtmr8 morphants show loss of arterial endothelial cell identity in dorsal aorta, which is effectively rescued by low concentration of PI3K inhibitor, and by over-expression of dnPKA mRNA or vegf mRNA. Interestingly, mtmr8 expression is up-regulated when zebrafish embryos are treated with specific inhibitor of Hedgehog pathway that abolishes arterial marker expression.
CONCLUSION: These data indicate that Mtmr8 is essential for vasculature development in zebrafish embryos, and may play a role in arterial specification through repressing PI3K activity. It is suggested that Mtmr8 should represent a novel element of the Hedgehog/PI3K/VEGF signaling cascade that controls arterial specification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping