PUBLICATION
Molecular cloning of IgT from Atlantic salmon, and analysis of the relative expression of tau, mu and delta in different tissues
- Authors
- Tadiso, T.M., Lie, K.K., and Hordvik, I.
- ID
- ZDB-PUB-100910-3
- Date
- 2011
- Source
- Veterinary Immunology and Immunopathology 139(1): 17-26 (Journal)
- Registered Authors
- Keywords
- IgT, IgM, IgD, Atlantic salmon, Teleost
- MeSH Terms
-
- Animals
- Brain/metabolism
- Cloning, Molecular
- DNA/genetics
- Gills/metabolism
- Immunoglobulin D/biosynthesis
- Immunoglobulin D/genetics
- Immunoglobulin Heavy Chains/biosynthesis
- Immunoglobulin Heavy Chains/genetics*
- Immunoglobulin M/biosynthesis
- Immunoglobulin M/genetics
- Immunoglobulin delta-Chains/biosynthesis
- Immunoglobulin delta-Chains/genetics*
- Immunoglobulin mu-Chains/biosynthesis
- Immunoglobulin mu-Chains/genetics*
- Kidney/metabolism
- Muscle, Skeletal/metabolism
- Polymerase Chain Reaction/veterinary
- RNA/genetics
- Salmo salar/genetics*
- Salmo salar/metabolism
- Sequence Alignment
- Sequence Analysis, DNA/veterinary
- Skin/metabolism
- Spleen/metabolism
- Thymus Gland/metabolism
- PubMed
- 20801526 Full text @ Vet. Immunol. Immunopathol.
Citation
Tadiso, T.M., Lie, K.K., and Hordvik, I. (2011) Molecular cloning of IgT from Atlantic salmon, and analysis of the relative expression of tau, mu and delta in different tissues. Veterinary Immunology and Immunopathology. 139(1):17-26.
Abstract
In the present study, IgT genes of Atlantic salmon were cloned and characterised. Analysis of our sequence data as well as ESTs reported to the databases revealed three distinct IgT heavy chain sub-variants in salmon, as opposed to two of IgM and IgD. The IgT sub-variants in salmon are 76-80% identical to each other, and 75-82% identical to the reported rainbow trout sequences, whereas the similarity to the orthologous molecules in zebrafish, grass carp, mandarin fish, and grouper is 25-41%. The heavy chains of both secreted and membrane anchored forms of salmon IgT include four constant Ig domains, tau1-tau4. This parallels the IgM heavy chains in elasmobranch fish and higher vertebrates, but differs from IgM in teleost fish where the membrane anchored form include only three constant Ig domains, mu1-mu3. The similarity between tau1 and mu1 in salmon is relatively high (52%) when compared to the remaining part of the molecules (tau2-tau4 and mu2-mu4 are 13-24% similar). To compare tau, mu and delta expressions in different tissues (head kidney, thymus, spleen, gill, skin, hind gut, brain and muscle) of Atlantic salmon, RT-qPCR assays were designed and evaluated. The analyses revealed that IgM transcripts are most abundant (up to 200 times more than IgD) followed by IgT (up to 20 times more than IgD) in most tissues. Highest expression of IgM, IgT, and IgD was in head kidney and spleen.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping