Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
- Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O.
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(10): 2603-2618 (Journal)
- Registered Authors
- Amsterdam, Adam, Barresi, Michael J. F., Hopkins, Nancy, Karlstrom, Rolf
- zebrafish, insertional mutants, axon guidance, astroglia, radial glia, glia, POC, chiasm, motor axon, hindbrain, neurogenesis, gliogenesis, Gfap
- MeSH Terms
- Animals, Genetically Modified
- Embryonic Development/genetics*
- Gene Expression Regulation, Developmental
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- 20806318 Full text @ Dev. Dyn.
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O. (2010) Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(10):2603-2618.
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes