|ZFIN ID: ZDB-PUB-100910-14|
Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O.
|Source:||Developmental dynamics : an official publication of the American Association of Anatomists 239(10): 2603-2618 (Journal)|
|Registered Authors:||Amsterdam, Adam, Barresi, Michael J. F., Hopkins, Nancy, Karlstrom, Rolf|
|Keywords:||zebrafish, insertional mutants, axon guidance, astroglia, radial glia, glia, POC, chiasm, motor axon, hindbrain, neurogenesis, gliogenesis, Gfap|
|PubMed:||20806318 Full text @ Dev. Dyn.|
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O. (2010) Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 239(10):2603-2618.
ABSTRACTThe formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.