PUBLICATION

Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis

Authors
Barresi, M.J., Burton, S., Dipietrantonio, K., Amsterdam, A., Hopkins, N., and Karlstrom, R.O.
ID
ZDB-PUB-100910-14
Date
2010
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   239(10): 2603-2618 (Journal)
Registered Authors
Amsterdam, Adam, Barresi, Michael J. F., Hopkins, Nancy, Karlstrom, Rolf
Keywords
zebrafish, insertional mutants, axon guidance, astroglia, radial glia, glia, POC, chiasm, motor axon, hindbrain, neurogenesis, gliogenesis, Gfap
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Astrocytes/cytology*
  • Astrocytes/metabolism*
  • Axons/metabolism*
  • Embryonic Development/genetics*
  • Gene Expression Regulation, Developmental
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
20806318 Full text @ Dev. Dyn.
Abstract
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a "shelf-screen" to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping