ZFIN ID: ZDB-PUB-100830-7
A Runx1 Intronic Enhancer Marks Hemogenic Endothelial Cells and Hematopoietic Stem Cells
Ng, C.E., Yokomizo, T., Yamashita, N., Cirovic, B., Jin, H., Wen, Z., Ito, Y., and Osato, M.
Date: 2010
Source: Stem Cells   28(10): 1869-1881 (Journal)
Registered Authors: Wen, Zilong
Keywords: hematopoietic stem cells, hemogenic endothelial cells, enhancer element, AML1, retrovirus integration site
MeSH Terms:
  • Animals
  • Base Sequence
  • Cell Line
  • Core Binding Factor Alpha 2 Subunit/genetics*
  • Enhancer Elements, Genetic/genetics*
  • Female
  • Flow Cytometry
  • Hemangioblasts/metabolism*
  • Hematopoietic Stem Cells/metabolism*
  • Male
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
PubMed: 20799333 Full text @ Stem Cells
ABSTRACT
Runx1 is essential for the generation of hematopoietic stem cells (HSCs) and is frequently mutated in human leukemias. However, the cis-regulatory mechanisms modulating the Runx1 gene expression remain to be elucidated. Herewith, we report the identification of an intronic Runx1 enhancer, Runx1 +24 mCNE, using a combinatorial in silico approach involving comparative genomics and retroviral integration sites mapping. The Runx1 +24 mCNE was found to possess hematopoietic-specific enhancer activity in both zebrafish and mouse models. Significantly, this enhancer is active specifically in hemogenic endothelial cells (ECs) at sites where the de novo generation of HSCs occurs. The activity of this enhancer is also strictly restricted to HSCs within the hematopoietic compartment of the adult bone marrow. We anticipate that Runx1 +24 mCNE HSC enhancer will serve as a molecular handle for tracing and/or manipulating hemogenic ECs/HSCs behavior in vivo and consequently become an invaluable tool for research on stem cell and cancer biology.
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