PUBLICATION
Developmental changes in the expression of sox2 in the zebrafish brain
- Authors
- Germanà, A., Montalbano, G., Guerrera, M.C., Amato, V., Laurà, R., Magnoli, D., Campo, S., Suarez-Fernandez, E., Ciriaco, E., and Vega, J.A.
- ID
- ZDB-PUB-100826-20
- Date
- 2011
- Source
- Microscopy research and technique 74(4): 347-54 (Journal)
- Registered Authors
- Amato, Valentina, Germanà, Antonino, Guerrera, Maria Cristina, Magnoli, Domenica, Montalbano, Giuseppe
- Keywords
- Sox2, brain, neurogenic zones, developmental changes, zebrafish
- MeSH Terms
-
- Animals
- Blotting, Western
- Brain/embryology*
- Brain Chemistry*
- Gene Expression Profiling*
- Gene Expression Regulation, Developmental*
- Immunohistochemistry
- Mammals
- Molecular Weight
- Real-Time Polymerase Chain Reaction
- SOX Transcription Factors/biosynthesis*
- Time Factors
- Zebrafish/embryology*
- Zebrafish Proteins/biosynthesis*
- PubMed
- 20734413 Full text @ Microsc. Res. Tech.
Citation
Germanà, A., Montalbano, G., Guerrera, M.C., Amato, V., Laurà, R., Magnoli, D., Campo, S., Suarez-Fernandez, E., Ciriaco, E., and Vega, J.A. (2011) Developmental changes in the expression of sox2 in the zebrafish brain. Microscopy research and technique. 74(4):347-54.
Abstract
The family of B1 Sox transcription factors plays critical roles in the early stages of development, including the central nervous system. It was demonstrated that Sox2 is expressed in repressed neural stem cells. Therefore, we decided to investigate the expression of Sox2 in the brain of zebrafish at different ages to identify potential neurogenic areas, and to establish the developmental changes they undergo. The brains were assessed by qRT-PCR, western blot, and immunohistochemistry. The maximal expression of Sox2 was found at 15 dpf progressively decreases up to 30 dpf, then increases up to 40 dpf and remains unchanged up to 180 dpf. By western blot three protein bands of 28 kDa, 34 kDa (main band), and 38 kDa were detected in the brain of 180 dpf animals. The immunolocalization of Sox2 revealed that by 15 dpf Sox2 was detected in cells of the olfactory bulb, the walls of the telencephalic and diencephalic ventricles, several nucleus in the diencephalons, and the tectum opticum; by 25-50 dpf the Sox2 positive areas were the same as above, and in the rhombencephalic ventricle and cerebellum. In adult animals Sox2 was restricted to the olfactory bulb and to cells of the telencephalic ventricle walls. Taken together present results demonstrate that the potential neurogenic areas in the brain of zebrafish are widespread than in mammals and change with development, but they are primarily concentrated around the ventricles and olfactory bulb in adults, following a similar localization as in mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping