PUBLICATION
Zebrafish (Danio rerio) vary by strain and sex in their behavioral and transcriptional responses to selenium supplementation
- Authors
- Benner, M.J., Drew, R.E., Hardy, R.W., and Robison, B.D.
- ID
- ZDB-PUB-100730-12
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 157(4): 310-318 (Journal)
- Registered Authors
- Keywords
- selenium, selenoproteins, zebrafish, gene expression, behavior, genotype by environment interaction
- MeSH Terms
-
- Analysis of Variance
- Animals
- Behavior, Animal/drug effects*
- Brain/drug effects
- Brain/enzymology
- Dietary Supplements*
- Female
- Gene Expression Regulation/drug effects
- Glutathione Peroxidase/metabolism
- Iodide Peroxidase/genetics
- Iodide Peroxidase/metabolism
- Male
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism
- Selenium/pharmacology*
- Selenoproteins/genetics
- Selenoproteins/metabolism
- Sex Characteristics*
- Species Specificity
- Thioredoxin Reductase 1/genetics
- Thioredoxin Reductase 1/metabolism
- Transcription, Genetic/drug effects*
- Zebrafish/classification
- Zebrafish/genetics*
- PubMed
- 20659579 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Benner, M.J., Drew, R.E., Hardy, R.W., and Robison, B.D. (2010) Zebrafish (Danio rerio) vary by strain and sex in their behavioral and transcriptional responses to selenium supplementation. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 157(4):310-318.
Abstract
We used the Nadia, Gaighatta, Scientific Hatcheries, and TM1 zebrafish (Danio rerio) strains to test the hypothesis that variation among populations influences the behavioral and transcriptional response to selenium supplementation. When fed a diet with control levels of selenium, zebrafish strains differed significantly in behavior, characterized as their mean horizontal and vertical swimming positions within the tank. The four strains also differed in brain expression of selenoprotein P1a (sepp1a), glutathione peroxidase 3 (gpx3), thioredoxin reductase 1 (txnrd1), and tRNA selenocysteine associated protein 1 (secp43). Iodothyronine deiodinase 2 (dio2) did not differ among strains but showed a sex-specific expression pattern. When supplemented with selenium, all strains spent a greater proportion of time near the front of the tank, but the response of vertical swimming depth varied by strain. Selenium supplementation also caused changes in selenoprotein expression in the brain that varied by strain for sepp1a, secp43, and dio2, and varied by strain and sex for txnrd1. Expression of gpx3 was unaffected by selenium. Our data indicate that selenium homeostasis in the brain may be a regulator of behavior in zebrafish, and the strain-specific effects of selenium supplementation suggest that genetic heterogeneity among populations can influence the results of selenium supplementation studies.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping