PUBLICATION
Characterization of the AP-1 mu1A and mu1B adaptins in zebrafish (Danio rerio)
- Authors
- Zizioli, D., Forlanelli, E., Guarienti, M., Nicoli, S., Fanzani, A., Bresciani, R., Borsani, G., Preti, A., Cotelli, F., and Schu, P.
- ID
- ZDB-PUB-100726-21
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(9): 2404-2412 (Journal)
- Registered Authors
- Borsani, Giuseppe, Cotelli, Franco, Nicoli, Stefania
- Keywords
- adaptor protein complexes, AP-1, clathrin, trans-Golgi network, membrane traffic, zebrafish, gut
- MeSH Terms
-
- Adaptor Protein Complex 1/genetics
- Adaptor Protein Complex 1/metabolism*
- Adaptor Protein Complex mu Subunits/classification
- Adaptor Protein Complex mu Subunits/genetics
- Adaptor Protein Complex mu Subunits/metabolism*
- Amino Acid Sequence
- Animals
- Gene Expression Regulation, Developmental
- Gene Knockdown Techniques
- Genome
- Humans
- Mice
- Molecular Sequence Data
- Morphogenesis/physiology*
- Phenotype
- Phylogeny
- Protein Isoforms/classification
- Protein Isoforms/genetics
- Protein Isoforms/metabolism*
- Sequence Alignment
- Tissue Distribution
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish Proteins/classification
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 20652956 Full text @ Dev. Dyn.
Citation
Zizioli, D., Forlanelli, E., Guarienti, M., Nicoli, S., Fanzani, A., Bresciani, R., Borsani, G., Preti, A., Cotelli, F., and Schu, P. (2010) Characterization of the AP-1 mu1A and mu1B adaptins in zebrafish (Danio rerio). Developmental Dynamics : an official publication of the American Association of Anatomists. 239(9):2404-2412.
Abstract
Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins gamma1, beta1, mu1, sigma1. Mammalia express mu1A ubiquitously and isoform mu1B in polarized epithelia. Mouse gamma1 or mu1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated mu1A and mu1B from Danio rerio. Analysis of mu1A and mu1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. mu1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. mu1B is not expressed in murine liver, indicating loss of mu1B expression and establishment of alternative sorting mechanisms during mammalian development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping