ZFIN ID: ZDB-PUB-100726-21
Characterization of the AP-1 mu1A and mu1B adaptins in zebrafish (Danio rerio)
Zizioli, D., Forlanelli, E., Guarienti, M., Nicoli, S., Fanzani, A., Bresciani, R., Borsani, G., Preti, A., Cotelli, F., and Schu, P.
Date: 2010
Source: Developmental dynamics : an official publication of the American Association of Anatomists 239(9): 2404-2412 (Journal)
Registered Authors: Borsani, Giuseppe, Cotelli, Franco, Nicoli, Stephania
Keywords: adaptor protein complexes, AP-1, clathrin, trans-Golgi network, membrane traffic, zebrafish, gut
MeSH Terms: Adaptor Protein Complex 1/genetics; Adaptor Protein Complex 1/metabolism*; Adaptor Protein Complex mu Subunits/classification; Adaptor Protein Complex mu Subunits/genetics; Adaptor Protein Complex mu Subunits/metabolism* (all 28) expand
PubMed: 20652956 Full text @ Dev. Dyn.
FIGURES   (current status)
ABSTRACT
Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins gamma1, beta1, mu1, sigma1. Mammalia express mu1A ubiquitously and isoform mu1B in polarized epithelia. Mouse gamma1 or mu1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated mu1A and mu1B from Danio rerio. Analysis of mu1A and mu1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. mu1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. mu1B is not expressed in murine liver, indicating loss of mu1B expression and establishment of alternative sorting mechanisms during mammalian development.
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