ZFIN ID: ZDB-PUB-100726-21
Characterization of the AP-1 mu1A and mu1B adaptins in zebrafish (Danio rerio)
Zizioli, D., Forlanelli, E., Guarienti, M., Nicoli, S., Fanzani, A., Bresciani, R., Borsani, G., Preti, A., Cotelli, F., and Schu, P.
Date: 2010
Source: Developmental dynamics : an official publication of the American Association of Anatomists 239(9): 2404-2412 (Journal)
Registered Authors: Borsani, Giuseppe, Cotelli, Franco, Nicoli, Stephania
Keywords: adaptor protein complexes, AP-1, clathrin, trans-Golgi network, membrane traffic, zebrafish, gut
MeSH Terms:
  • Adaptor Protein Complex 1/genetics
  • Adaptor Protein Complex 1/metabolism*
  • Adaptor Protein Complex mu Subunits/classification
  • Adaptor Protein Complex mu Subunits/genetics
  • Adaptor Protein Complex mu Subunits/metabolism*
  • Amino Acid Sequence
  • Animals
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Genome
  • Humans
  • Mice
  • Molecular Sequence Data
  • Morphogenesis/physiology*
  • Phenotype
  • Phylogeny
  • Protein Isoforms/classification
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism*
  • Sequence Alignment
  • Tissue Distribution
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 20652956 Full text @ Dev. Dyn.
FIGURES
ABSTRACT
Protein transport between the trans-Golgi network and endosomes is mediated by transport vesicles formed by the adaptor-protein complex AP-1, consisting of the adaptins gamma1, beta1, mu1, sigma1. Mammalia express mu1A ubiquitously and isoform mu1B in polarized epithelia. Mouse gamma1 or mu1A 'knock out's revealed that AP-1 is indispensable for embryonic development. We isolated mu1A and mu1B from Danio rerio. Analysis of mu1A and mu1B expression revealed tissue-specific expression for either one during embryogenesis and in adult tissues in contrast to their expression in mammalia. mu1B transcript was detected in organs of endodermal derivation and "knock-down" experiments gave rise to embryos defective in formation of intestine, liver, and pronephric ducts. Development ceased at 7-8 dpf. mu1B is not expressed in murine liver, indicating loss of mu1B expression and establishment of alternative sorting mechanisms during mammalian development.
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