High-throughput in vivo vertebrate screening
- Pardo-Martin, C., Chang, T.Y., Koo, B.K., Gilleland, C.L., Wasserman, S.C., and Yanik, M.F.
- Nature Methods 7(8): 634-636 (Journal)
- Registered Authors
- Yanik, Mehmet Faith
- MeSH Terms
- Embryo, Nonmammalian
- Equipment Design
- Laser Therapy
- Mass Screening/methods*
- Microscopy, Confocal/instrumentation*
- Models, Animal
- 20639868 Full text @ Nat. Methods
Pardo-Martin, C., Chang, T.Y., Koo, B.K., Gilleland, C.L., Wasserman, S.C., and Yanik, M.F. (2010) High-throughput in vivo vertebrate screening. Nature Methods. 7(8):634-636.
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Genes / Markers
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Engineered Foreign Genes
Errata and Notes