PUBLICATION

High-throughput in vivo vertebrate screening

Authors
Pardo-Martin, C., Chang, T.Y., Koo, B.K., Gilleland, C.L., Wasserman, S.C., and Yanik, M.F.
ID
ZDB-PUB-100719-67
Date
2010
Source
Nature Methods   7(8): 634-636 (Journal)
Registered Authors
Yanik, Mehmet Faith
Keywords
none
MeSH Terms
  • Animals
  • Automation
  • Axons*
  • Embryo, Nonmammalian
  • Equipment Design
  • Laser Therapy
  • Mass Screening/methods*
  • Microscopy, Confocal/instrumentation*
  • Models, Animal
  • Mutation
  • Retina/cytology*
  • Zebrafish
PubMed
20639868 Full text @ Nat. Methods
Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes