PUBLICATION
Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish
- Authors
- Francisco-Simão, M., Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F.
- ID
- ZDB-PUB-100625-20
- Date
- 2011
- Source
- Zygote (Cambridge, England) 19(1): 67-70 (Journal)
- Registered Authors
- Cardona-Costa, Jose
- Keywords
- Embryo, Germ-line chimaerism, Osmolarity, Zebrafish
- MeSH Terms
-
- Animals
- Osmolar Concentration
- Zebrafish/embryology*
- Chimerism/drug effects*
- Chimerism/embryology*
- Ultraviolet Rays*
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/radiation effects
- PubMed
- 20569510 Full text @ Zygote
Citation
Francisco-Simão, M., Cardona-Costa, J., Pérez-Camps, M., and García-Ximénez, F. (2011) Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish. Zygote (Cambridge, England). 19(1):67-70.
Abstract
The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping