ZFIN ID: ZDB-PUB-100614-28
Atoh8, a bHLH transcription factor, is required for the development of retina and skeletal muscle in zebrafish
Yao, J., Zhou, J., Liu, Q., Lu, D., Wang, L., Qiao, X., and Jia, W.
Date: 2010
Source: PLoS One   5(6): e10945 (Journal)
Registered Authors: Wang, Lu, Yao, Jihua
Keywords: Embryos, Zebrafish, Retina, Somites, Morpholino, Skeletal muscles, Neuronal differentiation, Muscle differentiation
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/chemistry
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/physiology*
  • Embryonic Development/physiology*
  • Gene Knockout Techniques
  • Humans
  • Molecular Sequence Data
  • Muscle, Skeletal/embryology*
  • Retina/embryology*
  • Sequence Homology, Amino Acid
  • Zebrafish/genetics*
PubMed: 20532172 Full text @ PLoS One
BACKGROUND: Math6/atoh8, a bHLH transcription factor, is thought to be indispensable for early embryonic development and likely has important roles in vertebrate tissue-specific differentiation. However, the function of Atoh8 during early development is not clear because homozygous knockout causes embryonic lethality in mice. We have examined the effects of the atoh8 gene on the differentiation of retina and skeletal muscle during early development in zebrafish. RESULTS: We isolated a Math6 homologue in zebrafish, designated as zebrafish atoh8. Whole -mount in situ hybridization analysis showed that zebrafish atoh8 is dynamically expressed mainly in developing retina and skeletal muscle. Atoh8-MO knock-down resulted in reduced eye size with disorganization of retinal lamination. The reduction of atoh8 function also affected the arrangement of paraxial cells and differentiated muscle fibers during somite morphogenesis. CONCLUSION: Our results show that Atoh8 is an important regulator for the development of both the retina and skeletal muscles necessary for neural retinal cell and myogenic differentiation during zebrafish embryogenesis.