PUBLICATION

Natural mixtures of persistent organic pollutants (POP) increase weight gain, advance puberty, and induce changes in gene expression associated with steroid hormones and obesity in female zebrafish

Authors
Lyche, J.L., Nourizadeh-Lillabadi, R., Almaas, C., Stavik, B., Berg, V., Skåre, J.U., Alestrøm, P., and Ropstad, E.
ID
ZDB-PUB-100614-19
Date
2010
Source
Journal of toxicology and environmental health. Part A   73(15): 1032-1057 (Journal)
Registered Authors
Nourizadeh-Lillabadi, Rasoul
Keywords
none
MeSH Terms
  • Organic Chemicals/analysis
  • Organic Chemicals/toxicity*
  • RNA/biosynthesis
  • RNA/genetics
  • Zebrafish
  • Fresh Water
  • Gene Expression/drug effects*
  • Sexual Maturation/drug effects*
  • Complex Mixtures/analysis
  • Complex Mixtures/toxicity*
  • Artemia/chemistry
  • Water Pollutants, Chemical/analysis
  • Water Pollutants, Chemical/toxicity*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Gene Expression Profiling
  • In Situ Hybridization
  • DDT/analysis
  • DDT/toxicity
  • Obesity/chemically induced*
  • Polychlorinated Biphenyls/analysis
  • Polychlorinated Biphenyls/toxicity
  • Sex Ratio
  • Weight Gain/drug effects*
  • Animals
  • Halogenated Diphenyl Ethers/analysis
  • Halogenated Diphenyl Ethers/toxicity
  • Female
  • Steroids/biosynthesis*
  • Liver/chemistry
  • Liver/metabolism
  • Oligonucleotide Array Sequence Analysis
(all 31)
PubMed
20526952 Full text @ J. Toxicol. Environ. Health. A.
CTD
20526952
Abstract
In the present study, developmental and reproductive effects of lifelong exposure to environmental relevant concentrations of two natural mixtures of persistent organic pollutants (POP) were investigated using classical and molecular methods in a controlled zebrafish model. The mixtures used were extracted from burbot (Lota lota) liver originating from freshwater systems in Norway: one mixture with high levels and one mixture with background levels of polybrominated diphenyl ethers (PBDE), polychlorinated biphenyls (PCB), and dichlorodiphenyltrichloroethane metabolites (DDT). The concentration of POP measured in the zebrafish ranged from levels detected in wild fish from Lake Mjøsa to concentrations reported in human and wildlife populations, indicating that the experimental fish were exposed to concentrations comparable with wild fish. Phenotypic effects observed in both exposure groups included earlier onset of puberty, increased male/female sex ratio, and differences in body weight at 5 mo of age. Interestingly, genome-wide transcription profiling showed changes in regulation of genes involved in endocrine signaling and growth. The transcriptomics changes include key regulator genes for steroid hormone functions (ncoa3), and growth (c/ebp, ncoa3). The effects observed in the experimental zebrafish model raise the question whether chemical pollution represents a risk to reproductive health of wild fish inhabitating the freshwater system.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Lyche, J.L., Nourizadeh-Lillabadi, R., Almaas, C., Stavik, B., Berg, V., Skåre, J.U., Alestrøm, P., and Ropstad, E. (2010) Natural mixtures of persistent organic pollutants (POP) increase weight gain, advance puberty, and induce changes in gene expression associated with steroid hormones and obesity in female zebrafish. Journal of toxicology and environmental health. Part A. 73(15):1032-1057.