ZFIN ID: ZDB-PUB-100601-11
Expression of the zebrafish CD133/prominin1 genes in cellular proliferation zones in the embryonic central nervous system and sensory organs
McGrail, M., Batz, L., Noack, K., Pandey, S., Huang, Y., Gu, X., and Essner, J.J.
Date: 2010
Source: Developmental dynamics : an official publication of the American Association of Anatomists   239(6): 1849-1857 (Journal)
Registered Authors: Essner, Jeffrey, McGrail, Maura
Keywords: proliferating cells, retinal epithelium, neural retina, dorsal tectum, ventricular zone, epiphysis, rhombomere boundaries
MeSH Terms:
  • Animals
  • Antigens, CD
  • Cell Proliferation*
  • Central Nervous System/embryology*
  • Central Nervous System/metabolism*
  • Embryo, Nonmammalian
  • Embryonic Development/genetics
  • Genes
  • Glycoproteins
  • Peptides
  • Sense Organs/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 20503380 Full text @ Dev. Dyn.
The CD133/prominin1 gene encodes a pentamembrane glycoprotein cell surface marker that is expressed in stem cells from neuroepithelial, hematopoietic, and various organ tissues. Here we report the analysis of two zebrafish CD133/prominin1 orthologues, prominin1a and prominin1b. The expression patterns of the zebrafish prominin1a and b genes were analyzed during embryogenesis using whole mount in situ hybridization. prominin1a and b show novel complementary and overlapping patterns of expression in proliferating zones in the developing sensory organs and central nervous system. The expression patterns suggest functional conservation of the zebrafish prominin1 genes. Initial analyses of prominin1a and b in neoplastic tissue show increased expression of both genes in a subpopulation of cells in malignant peripheral nerve sheath tumors in tp53 mutants. Based on these analyses, the zebrafish prominin1 genes will be useful markers for examining proliferating cell populations in adult organs, tissues, and tumors.