PUBLICATION
Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model
- Authors
- Alex, D., Lam, I.K., Lin, Z., and Lee, S.M.
- ID
- ZDB-PUB-100525-12
- Date
- 2010
- Source
- Journal of ethnopharmacology 131(2): 242-247 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Angiogenesis Inhibitors/pharmacology*
- Animals
- Animals, Genetically Modified
- Apoptosis/drug effects
- Cell Cycle/drug effects
- Cell Line
- Cell Proliferation/drug effects
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Drugs, Chinese Herbal/pharmacology*
- Endothelial Cells/drug effects*
- Endothelium, Vascular/drug effects*
- Humans
- In Situ Nick-End Labeling
- Indoles/pharmacology
- Magnoliopsida*
- Umbilical Veins/cytology
- Umbilical Veins/drug effects
- Zebrafish/embryology
- PubMed
- 20488232 Full text @ J. Ethnopharmacol.
Citation
Alex, D., Lam, I.K., Lin, Z., and Lee, S.M. (2010) Indirubin shows anti-angiogenic activity in an in vivo zebrafish model and an in vitro HUVEC model. Journal of ethnopharmacology. 131(2):242-247.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: Indirubin is an active ingredient of the traditional Chinese medicine, Dang Gui Long Hui Wan, commonly used for the treatment of chronic myelocytic leukemia (CML) and other inflammatory conditions. These anti-leukemic and anti-inflammatory activities may be mediated by anti-angiogenic action and valuable for the treatment of diseases with excessive angiogenesis such as CML and psoriasis. AIM OF THE STUDY: To investigate the anti-angiogenic activity of indirubin, we tested its inhibitory effect on blood vessel formation in zebrafish embryos and on endothelial cell proliferation in culture. MATERIALS AND METHODS: The anti-angiogenic activity of indirubin was tested using transgenic zebrafish embryos with fluorescent vasculature and human umbilical vein endothelial cells (HUVECs). Apoptosis was analyzed with a Terminal Deoxynucleotidyl Transferase Mediated dUTP Nick End Labeling (TUNEL) assay. RESULTS: Indirubin dose-dependently inhibited intersegmental vessel formation in zebrafish embryos. It also inhibited HUVEC proliferation by the induction of cellular apoptosis and cell-cycle arrest at the G(0)/G(1) phase. CONCLUSIONS: The anti-angiogenic activity of indirubin may partly contribute to its anti-leukemic and anti-psoriatic properties and may be valuable for the treatment of diseases with excessive angiogenesis. The zebrafish model of angiogenesis was further validated in this study.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping