PUBLICATION

Zebrafish-Encoded 3-O-Sulfotransferase-3 Isoform Mediates Herpes Simplex Virus Type 1 Entry and Spread

Authors
Hubbard, S., Darmani, N.A., Thrush, G.R., Dey, D., Burnham, L., Thompson, J.M., Jones, K., and Tiwari, V.
ID
ZDB-PUB-100511-6
Date
2010
Source
Zebrafish   7(2): 181-187 (Journal)
Registered Authors
Tiwari, Vaibhav
Keywords
none
MeSH Terms
  • CHO Cells
  • Heparan Sulfate Proteoglycans/metabolism
  • Sulfotransferases/metabolism*
  • Protein Isoforms/metabolism
  • Herpes Simplex/enzymology*
  • Zebrafish/metabolism*
  • Zebrafish/virology*
  • Cricetulus
  • Membrane Fusion/physiology*
  • Virus Internalization
  • Cricetinae
  • Virus Replication/physiology*
  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Herpesvirus 1, Human*
  • Microscopy, Fluorescence
  • RNA Interference
(all 17)
PubMed
20441522 Full text @ Zebrafish
Abstract
Heparan sulfate proteoglycans modified by human glucosaminyl 3-O-sulfotransferase-3 (3-OST-3) isoform generates the cellular receptor for herpes simplex virus type 1 (HSV-1). Interestingly, the ability of zebrafish (ZF)-encoded 3-OST-3 isoform to modify heparan sulfate to mediate HSV-1 entry and cell-cell fusion has not been determined although it is predominantly expressed in ZF, a popular model organism to study viral infections. Here, we demonstrate that expression of ZF-encoded 3-OST-3 isoform renders the resistant Chinese hamster ovary (CHO-K1) cells to become susceptible for HSV-1 entry. The following lines of evidence support the important role of ZF-encoded 3-OST-3 isoform as the mediator of HSV-1 entry into CHO-K1 cells: (1) ZF 3-OST-3-expressing CHO-K1 cells were able to preferentially bind HSV-1 glycoprotein D, and (2) CHO-K1 cells expressing ZF-encoded 3-OST-3 acquire the ability to fuse with cells expressing HSV-1 glycoproteins. Finally, knocking down 3-OST-3 receptor by siRNA in ZF fibroblasts cells significantly reduced HSV-1 entry and glycoprotein D binding to cells. Taken together, our results provide novel insight into the significance of ZF 3-OST-3 isoform as an HSV-1 entry and fusion receptor and its potential involvement in the HSV-1 disease model of ZF.
Genes / Markers
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Fish
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Citation
Hubbard, S., Darmani, N.A., Thrush, G.R., Dey, D., Burnham, L., Thompson, J.M., Jones, K., and Tiwari, V. (2010) Zebrafish-Encoded 3-O-Sulfotransferase-3 Isoform Mediates Herpes Simplex Virus Type 1 Entry and Spread. Zebrafish. 7(2):181-187.