PUBLICATION
Zebrafish-Encoded 3-O-Sulfotransferase-3 Isoform Mediates Herpes Simplex Virus Type 1 Entry and Spread
- Authors
- Hubbard, S., Darmani, N.A., Thrush, G.R., Dey, D., Burnham, L., Thompson, J.M., Jones, K., and Tiwari, V.
- ID
- ZDB-PUB-100511-6
- Date
- 2010
- Source
- Zebrafish 7(2): 181-187 (Journal)
- Registered Authors
- Tiwari, Vaibhav
- Keywords
- none
- MeSH Terms
-
- Animals
- CHO Cells
- Cricetinae
- Cricetulus
- Enzyme-Linked Immunosorbent Assay
- Heparan Sulfate Proteoglycans/metabolism
- Herpes Simplex/enzymology*
- Herpesvirus 1, Human*
- Membrane Fusion/physiology*
- Microscopy, Fluorescence
- Protein Isoforms/metabolism
- RNA Interference
- Sulfotransferases/metabolism*
- Virus Internalization
- Virus Replication/physiology*
- Zebrafish/metabolism*
- Zebrafish/virology*
- PubMed
- 20441522 Full text @ Zebrafish
Citation
Hubbard, S., Darmani, N.A., Thrush, G.R., Dey, D., Burnham, L., Thompson, J.M., Jones, K., and Tiwari, V. (2010) Zebrafish-Encoded 3-O-Sulfotransferase-3 Isoform Mediates Herpes Simplex Virus Type 1 Entry and Spread. Zebrafish. 7(2):181-187.
Abstract
Heparan sulfate proteoglycans modified by human glucosaminyl 3-O-sulfotransferase-3 (3-OST-3) isoform generates the cellular receptor for herpes simplex virus type 1 (HSV-1). Interestingly, the ability of zebrafish (ZF)-encoded 3-OST-3 isoform to modify heparan sulfate to mediate HSV-1 entry and cell-cell fusion has not been determined although it is predominantly expressed in ZF, a popular model organism to study viral infections. Here, we demonstrate that expression of ZF-encoded 3-OST-3 isoform renders the resistant Chinese hamster ovary (CHO-K1) cells to become susceptible for HSV-1 entry. The following lines of evidence support the important role of ZF-encoded 3-OST-3 isoform as the mediator of HSV-1 entry into CHO-K1 cells: (1) ZF 3-OST-3-expressing CHO-K1 cells were able to preferentially bind HSV-1 glycoprotein D, and (2) CHO-K1 cells expressing ZF-encoded 3-OST-3 acquire the ability to fuse with cells expressing HSV-1 glycoproteins. Finally, knocking down 3-OST-3 receptor by siRNA in ZF fibroblasts cells significantly reduced HSV-1 entry and glycoprotein D binding to cells. Taken together, our results provide novel insight into the significance of ZF 3-OST-3 isoform as an HSV-1 entry and fusion receptor and its potential involvement in the HSV-1 disease model of ZF.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping