PUBLICATION
Development of in vitro culture method for early stage zebrafish (Danio rerio) ovarian follicles for use in cryopreservation studies
- Authors
- Tsai, S., Rawson, D.M., and Zhang, T.
- ID
- ZDB-PUB-100511-20
- Date
- 2010
- Source
- Theriogenology 74(2): 290-303 (Journal)
- Registered Authors
- Rawson, David M.
- Keywords
- Zebrafish, Ovarian follicle, in vitro, hCG, Activin A, Cryopreservation
- MeSH Terms
-
- Activins/pharmacology
- Animals
- Cell Culture Techniques*
- Chorionic Gonadotropin/pharmacology
- Cryopreservation/veterinary*
- Culture Media
- Female
- Hydrogen-Ion Concentration
- Ovarian Follicle/cytology*
- Ovarian Follicle/drug effects
- Ovarian Follicle/growth & development
- Reproductive Control Agents/pharmacology
- Zebrafish/growth & development*
- PubMed
- 20452004 Full text @ Theriogenology
Citation
Tsai, S., Rawson, D.M., and Zhang, T. (2010) Development of in vitro culture method for early stage zebrafish (Danio rerio) ovarian follicles for use in cryopreservation studies. Theriogenology. 74(2):290-303.
Abstract
There have been no reported methods for in vitro growth of early stage ovarian follicles for fish and their cryopreservation is still under investigation. If cryopreservation of early stage ovarian follicles can be achieved, in vitro procedures for ovarian follicle culture, development, ovulation and fertilisation after cryopreservation would be needed. The aim of the present study was to develop an in vitro culture method for early stage zebrafish ovarian follicles for use after their cryopreservation. Procedures for in vitro culture of stage I (primary growth) and stage II (cortical alveolus) ovarian follicles were developed. The effects of concentration of L-15 medium, pH and the concentration of human chorionic gonadotropin (hCG) and activin A were studied. The results demonstrated that early stage zebrafish ovarian follicles can be cultured in vitro for 24 h, stage I and II ovarian follicles can grow to the sizes of early stage II and stage III ovarian follicles after hCG treatment. The method developed here is effective for assessing early stage zebrafish ovarian follicles growth competence in vitro. The results from the present study indicated that in vitro culture is the most reliable method for assessing ovarian follicle viability when compared with vital dye staining methods.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping