PUBLICATION
The role of the insulin-like growth factor (IGF) system in zebrafish (Danio rerio) ovarian development
- Authors
- Nelson, S.N., and Van Der Kraak, G.
- ID
- ZDB-PUB-100511-17
- Date
- 2010
- Source
- General and comparative endocrinology 168(1): 103-110 (Journal)
- Registered Authors
- Van Der Kraak, Glen
- Keywords
- Insulin-like growth factor, Zebrafish, Oocyte maturation, Maturational competence, Ovarian development, Steroidogenesis
- MeSH Terms
-
- Animals
- Chorionic Gonadotropin/pharmacology
- Female
- Oocytes/drug effects*
- Ovary/drug effects*
- Ovary/growth & development*
- Reverse Transcriptase Polymerase Chain Reaction
- Somatomedins/pharmacology*
- Zebrafish/growth & development*
- PubMed
- 20447401 Full text @ Gen. Comp. Endocrinol.
Citation
Nelson, S.N., and Van Der Kraak, G. (2010) The role of the insulin-like growth factor (IGF) system in zebrafish (Danio rerio) ovarian development. General and comparative endocrinology. 168(1):103-110.
Abstract
The presence of an ovarian IGF system in teleosts suggests a distinct role in reproductive physiology. This study investigates the role of the ovarian IGF system in oocyte maturation, the acquisition of maturational competence and steroidogenesis in the zebrafish (Danio rerio). Recombinant human IGF-I and IGF-II stimulated germinal vesicle breakdown (GVBD) in early vitellogenic (EV; 0.35-0.44 mm), midvitellogenic (MV; 0.45-0.56 mm) and full grown (FG; 0.57-0.65 mm) follicles incubated in vitro. By comparison, the maturation inducing steroid 17alpha,20beta-dihydroxy-4-pregnen-3-one (17,20beta-P) only induced GVBD in MV and FG follicles. Collectively these studies suggest that IGF is involved in oocyte maturation and that follicles become responsive to IGFs at an earlier stage compared to 17,20beta-P. IGF-I also increased the responsiveness of the follicle to 17,20beta-P, suggesting a role in promoting maturational competence. IGF-I alone and in combination with human chorionic gonadotropin (hCG) stimulated the production of 17,20beta-P by ovarian follicles incubated in vitro. However, IGF-I had no effect on the production of 17beta-estradiol (E(2)) or the expression of genes involved in steroidogenesis (20beta-hydroxysteroid dehydrogenase; 20beta-HSD and P450c17-II). These results provide evidence that the IGF system plays an important role in the promotion of oocyte maturation and ovarian development in the zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping