ZFIN ID: ZDB-PUB-100504-2
Visualizing Voltage Dynamics in Zebrafish Heart
Tsutsui, H., Higashijima, S.I., Miyawaki, A., and Okamura, Y.
Date: 2010
Source: The Journal of physiology   588(Pt 12): 2017-2021 (Journal)
Registered Authors: Higashijima, Shin-ichi
Keywords: Electrical activity, Fluroscence, Imaging
MeSH Terms:
  • Action Potentials
  • Animals
  • Animals, Genetically Modified
  • Anti-Allergic Agents/toxicity
  • Astemizole/toxicity
  • Biosensing Techniques
  • Cardiac Myosins/genetics
  • Dose-Response Relationship, Drug
  • Ether-A-Go-Go Potassium Channels/antagonists & inhibitors
  • Ether-A-Go-Go Potassium Channels/metabolism
  • Excitation Contraction Coupling/drug effects
  • Fluorescence Recovery After Photobleaching
  • Heart Rate/drug effects
  • Kinetics
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Microscopy, Fluorescence
  • Myocytes, Cardiac/drug effects
  • Myocytes, Cardiac/metabolism*
  • Myosin Light Chains/genetics
  • Promoter Regions, Genetic
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/metabolism
PubMed: 20421282 Full text @ J. Physiol.
ABSTRACT
The zebrafish heart provides a useful vertebrate cardiovascular model with outstanding advantages, including genetic manipulatability, optical accessibility and rapid development. In addition, an emerging topic in cardiotoxicity assay and drug discovery is its use in phenotype-based chemical screen. Here, we report a technique that allows non-invasive voltage mapping in beating heart using a genetically encoded probe for transmembrane potential. Application of anti-allergy compound, astemizole, resulted in aberrant propagation of excitation, which accounted for the lack of ventricular contraction. This optical method will provide new opportunities in broad areas of physiological, developmental, and pharmacological cardiovascular research.
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