PUBLICATION

Visualizing Voltage Dynamics in Zebrafish Heart

Authors
Tsutsui, H., Higashijima, S.I., Miyawaki, A., and Okamura, Y.
ID
ZDB-PUB-100504-2
Date
2010
Source
The Journal of physiology   588(Pt 12): 2017-2021 (Journal)
Registered Authors
Higashijima, Shin-ichi
Keywords
Electrical activity, Fluroscence, Imaging
MeSH Terms
  • Biosensing Techniques
  • Promoter Regions, Genetic
  • Luminescent Proteins/biosynthesis
  • Luminescent Proteins/genetics
  • Myosin Light Chains/genetics
  • Excitation Contraction Coupling/drug effects
  • Animals, Genetically Modified
  • Cardiac Myosins/genetics
  • Dose-Response Relationship, Drug
  • Anti-Allergic Agents/toxicity
  • Animals
  • Microscopy, Fluorescence
  • Myocytes, Cardiac/drug effects
  • Myocytes, Cardiac/metabolism*
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/metabolism
  • Heart Rate/drug effects
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Fluorescence Recovery After Photobleaching
  • Ether-A-Go-Go Potassium Channels/antagonists & inhibitors
  • Ether-A-Go-Go Potassium Channels/metabolism
  • Kinetics
  • Action Potentials
  • Astemizole/toxicity
PubMed
20421282 Full text @ J. Physiol.
Abstract
The zebrafish heart provides a useful vertebrate cardiovascular model with outstanding advantages, including genetic manipulatability, optical accessibility and rapid development. In addition, an emerging topic in cardiotoxicity assay and drug discovery is its use in phenotype-based chemical screen. Here, we report a technique that allows non-invasive voltage mapping in beating heart using a genetically encoded probe for transmembrane potential. Application of anti-allergy compound, astemizole, resulted in aberrant propagation of excitation, which accounted for the lack of ventricular contraction. This optical method will provide new opportunities in broad areas of physiological, developmental, and pharmacological cardiovascular research.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping