PUBLICATION
Identification of residues critical for the cell-membrane-penetrating activity of zebrafish neuroglobin
- Authors
- Watanabe, S., and Wakasugi, K.
- ID
- ZDB-PUB-100427-23
- Date
- 2010
- Source
- FEBS letters 584(11): 2467-2472 (Journal)
- Registered Authors
- Wakasugi, Keisuke
- Keywords
- Neuroglobin, Fish globin, Cell-membrane-penetrating protein, Protein transduction
- MeSH Terms
-
- Cell Membrane/metabolism*
- Nerve Tissue Proteins/chemistry*
- Nerve Tissue Proteins/metabolism
- Cells/metabolism
- Fishes/metabolism
- Mutagenesis, Site-Directed
- Zebrafish/metabolism*
- Animals
- Globins/chemistry*
- Globins/metabolism
- Cellular Structures/metabolism*
- PubMed
- 20417633 Full text @ FEBS Lett.
Citation
Watanabe, S., and Wakasugi, K. (2010) Identification of residues critical for the cell-membrane-penetrating activity of zebrafish neuroglobin. FEBS letters. 584(11):2467-2472.
Abstract
Neuroglobin (Ngb) is a globin found in the vertebrate brain. Recently, we found that zebrafish Ngb can translocate into cells and clarified that module M1 of zebrafish Ngb is important for protein transduction. In the present study, we used site-directed mutagenesis to identify residues of module M1 that are important for protein transduction. We show that Lys7, Lys9, Lys21, and Lys23 of zebrafish Ngb are crucial for its activity. Since these residues are conserved among fishes, but not among mammals, birds, or amphibians, the ability to penetrate cell membranes may be a unique characteristic of fish Ngb proteins.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping