PUBLICATION
Proteomics Profiling of Single Organs from Individual Adult Zebrafish
- Authors
- Abramsson, A., Westman-Brinkmalm, A., Pannee, J., Gustavsson, M., von Otter, M., Blennow, K., Brinkmalm, G., Kettunen, P., and Zetterberg, H.
- ID
- ZDB-PUB-100420-33
- Date
- 2010
- Source
- Zebrafish 7(2): 161-168 (Journal)
- Registered Authors
- Kettunen, Petronella, Zetterberg, Henrik
- Keywords
- none
- MeSH Terms
-
- Animals
- Brain/metabolism
- Chromatography, Liquid
- Gene Expression Profiling/methods*
- Gene Expression Regulation/genetics*
- Gene Regulatory Networks/genetics*
- Intestines/metabolism
- Liver/metabolism
- Muscle, Skeletal/metabolism
- Myocardium/metabolism
- Proteins/metabolism*
- Proteomics/methods*
- Tandem Mass Spectrometry
- Zebrafish/metabolism*
- PubMed
- 20392139 Full text @ Zebrafish
Citation
Abramsson, A., Westman-Brinkmalm, A., Pannee, J., Gustavsson, M., von Otter, M., Blennow, K., Brinkmalm, G., Kettunen, P., and Zetterberg, H. (2010) Proteomics Profiling of Single Organs from Individual Adult Zebrafish. Zebrafish. 7(2):161-168.
Abstract
The model organism zebrafish (Danio rerio) is extensively utilized in studies of developmental biology but is also being investigated in the context of a growing list of human age-related diseases. To facilitate such studies, we here present protein expression patterns of adult zebrafish organs, including blood, brain, fin, heart, intestine, liver, and skeletal muscle. Protein extracts were prepared from the different organs of two zebrafish and analyzed using liquid chromatography coupled to high-resolution tandem mass spectrometry. Zebrafish tissue was digested directly after minimal fractionation and cleaned up (the shotgun approach). Proteins were identified using Mascot software. In total, 1394 proteins were identified of which 644 were nonredundant. Of these, 373 demonstrated an organ-specific expression pattern and 57 had not been shown on protein level before. These data emphasize the need for increased research at the protein level to facilitate the selection of candidate proteins for targeted quantification and to refine systematic genetic network analysis in vertebrate development, biology, and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping