ZFIN ID: ZDB-PUB-100420-32
Oligonucleotide-Based Tools for Studying Zebrafish Development
Shestopalov, I.A., and Chen, J.K.
Date: 2010
Source: Zebrafish   7(1): 31-40 (Review)
Registered Authors: Chen, James K., Shestopalov, Ilya
Keywords: none
MeSH Terms:
  • Animals
  • Gene Expression Regulation, Developmental
  • Genetic Techniques*
  • Oligonucleotides/genetics*
  • RNA/genetics
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
PubMed: 20392138 Full text @ Zebrafish
ABSTRACT
Synthetic and nonnatural oligonucleotides have been used extensively to interrogate gene function in zebrafish. In this review, we survey the capabilities and limitations of various oligonucleotide-based technologies for perturbing RNA function and tracking RNA expression. We also examine recent strategies for achieving spatiotemporal control of oligonucleotide function, particularly light-gated technologies that exploit the optical transparency of zebrafish embryos.
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