PUBLICATION
Zebrafish 10-formyltetrahydrofolate dehydrogenase is similar to its mammalian isozymes for its structural and catalytic properties
- Authors
- Chang, W.N., Lin, H.C., and Fu, T.F.
- ID
- ZDB-PUB-100420-10
- Date
- 2010
- Source
- Protein Expression and Purification 72(2): 217-222 (Journal)
- Registered Authors
- Chang, Wen-Ni, Fu, Tzu-Fun
- Keywords
- 10-Formyltetrahydrofolate dehydrogenase, Folate, Folic acid, Folate-mediated one-carbon metabolism, Zebrafish, Enzyme
- MeSH Terms
-
- Aldehydes
- Animals
- Cell Line
- Chromatography, Affinity
- Chromatography, Gel
- Cloning, Molecular
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli/genetics
- Female
- Humans
- Isoenzymes/chemistry
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Leucovorin/analogs & derivatives
- Leucovorin/metabolism
- Male
- Oxidoreductases Acting on CH-NH Group Donors/chemistry*
- Oxidoreductases Acting on CH-NH Group Donors/genetics
- Oxidoreductases Acting on CH-NH Group Donors/metabolism*
- Pichia/genetics
- Protein Structure, Quaternary
- Protein Structure, Tertiary
- Recombinant Proteins/chemistry
- Recombinant Proteins/genetics
- Recombinant Proteins/metabolism
- Zebrafish
- PubMed
- 20381623 Full text @ Protein Expr. Purif.
Citation
Chang, W.N., Lin, H.C., and Fu, T.F. (2010) Zebrafish 10-formyltetrahydrofolate dehydrogenase is similar to its mammalian isozymes for its structural and catalytic properties. Protein Expression and Purification. 72(2):217-222.
Abstract
10-Formyltetrahydrofolate dehydrogenase from zebrafish has been cloned and expressed in both Escherichia coli and yeast. In addition, the N-terminal and C-terminal domains have also been cloned and expressed. Each expressed protein was purified to homogeneity and structural and kinetic properties determined. These studies show that the zebrafish enzyme is structurally and catalytically very similar to the enzymes from mammalian sources, suggesting that zebrafish can be used to study the in vivo function of 10-formyltetrahydrofolate dehydrogenase.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping