PUBLICATION

Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation

Authors
Paul, T.A., Rovnak, J., Quackenbush, S.L., Whitlock, K., Zhan, H., Gong, Z., Spitsbergen, J., Bowser, P.R., and Casey, J.W.
ID
ZDB-PUB-100330-43
Date
2011
Source
Marine biotechnology (New York, N.Y.)   13(2): 142-150 (Journal)
Registered Authors
Bowser, Paul R., Gong, Zhiyuan, Paul, Thomas A., Spitsbergen, Jan, Whitlock, Kate, Zhan, Huiqing
Keywords
Walleye dermal sarcoma virus, orfA, rv-cyclin, Fish retrovirus, Walleye dermal sarcoma, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified/metabolism*
  • Cell Proliferation
  • Epsilonretrovirus/genetics*
  • Fish Diseases/metabolism*
  • Fish Diseases/pathology
  • Fish Diseases/virology
  • Gene Expression Regulation, Viral
  • Gene Transfer Techniques
  • Genes, Viral
  • Regeneration/genetics
  • Sarcoma/metabolism
  • Sarcoma/pathology
  • Sarcoma/veterinary*
  • Sarcoma/virology
  • Skin Neoplasms/metabolism
  • Skin Neoplasms/pathology
  • Skin Neoplasms/veterinary*
  • Skin Neoplasms/virology
  • Tail/injuries
  • Tail/metabolism
  • Viral Proteins/genetics
  • Viral Proteins/metabolism
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
20349325 Full text @ Mar. Biotechnol.
Abstract
Walleye dermal sarcoma (WDS) is a benign tumor of walleye fish that develops and completely regresses seasonally. The retrovirus associated with this disease, walleye dermal sarcoma virus, encodes three accessory genes, two of which, rv-cyclin (orfA) and orfb, are thought to play a role in tumor development. In this study, we attempted to recapitulate WDS development by expressing rv-cyclin in chimeric and stable transgenic zebrafish. Six stable transgenic lines expressing rv-cyclin from the constitutive CMVtk promoter were generated. Immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction demonstrate that rv-cyclin is widely expressed in different tissues in these fish. These lines were viable and histologically normal for up to 2 years. No increase in tumors or tissue proliferation was observed following N-ethyl N-nitrosourea exposure or following tail wounding and subsequent tissue regeneration compared to controls. These data indicate that rv-cyclin is not independently sufficient for tumor induction in zebrafish.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes