PUBLICATION

Characterisation of expression patterns and functional role of Cactin in early zebrafish development

Authors
Atzei, P., Yang, F., Collery, R., Kennedy, B.N., and Moynagh, P.N.
ID
ZDB-PUB-100330-40
Date
2010
Source
Gene expression patterns : GEP   10(4-5): 199-206 (Journal)
Registered Authors
Collery, Ross, Kennedy, Breandan N.
Keywords
Toll-like Receptors, NF-κB, Cactin, early development, In situ hybridisation, morpholino
MeSH Terms
  • Animals
  • Base Sequence
  • Carrier Proteins/genetics*
  • DNA Primers
  • Drosophila Proteins/genetics*
  • Gene Expression Regulation, Developmental*
  • Gene Knockdown Techniques
  • Humans
  • Polymerase Chain Reaction
  • Zebrafish/embryology*
PubMed
20348034 Full text @ Gene Expr. Patterns
Abstract
The immune system of teleost zebrafish (Danio rerio) shows high similarity to mammalian counterparts sharing many innate immune components including Toll-Like Receptors (TLRs), cytokines, chemokines and complement molecules. As in mammals, zebrafish also contains the transcription factor NF-kappaB that plays dualist roles in innate immunity and early development. Indeed NF-kappaB members are expressed in different temporal patterns during the early stages of zebrafish embryogenesis indicating that each molecule is involved in specific developmental events. In the present study we employ zebrafish as a model to characterise the expression pattern and role of a novel NF-kappaB regulator, termed Cactin, in early development. Cactin was first characterised in Drosophila as a new member of the Rel pathway that could affect the generation of dorsal-ventral polarity. To explore the potential developmental role of Cactin in zebrafish, we initially investigated its expression pattern and functional role during early embryonic developmental stages. We detect Cactin expression at all stages of early development and knockdown of Cactin by specific morpholino antisense oligonucleotides causes developmental abnormalities manifested by an overall dysmorphic cellular organization. These results indicate that Cactin has been highly conserved during evolution and plays a key role in early embryonic development.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping