Voltage-Gated Sodium Channels Are Required for Heart Development in Zebrafish
- Chopra, S.S., Stroud, D.M., Watanabe, H., Bennett, J.S., Burns, C.G., Wells, K.S., Yang, T., Zhong, T.P., and Roden, D.M.
- Circulation research 106(8): 1342-1350 (Journal)
- Registered Authors
- Zhong, Tao P.
- ion channels, heart development, zebrafish, scn5La
- MeSH Terms
- Age Factors
- Amino Acid Sequence
- Animals, Genetically Modified
- Basic Helix-Loop-Helix Transcription Factors/genetics
- CHO Cells
- Cell Differentiation
- GATA Transcription Factors/genetics
- Gene Expression Regulation, Developmental*
- Gene Knockdown Techniques
- Heart Defects, Congenital/embryology
- Heart Defects, Congenital/genetics*
- Heart Defects, Congenital/metabolism
- Membrane Potentials
- Molecular Sequence Data
- NAV1.5 Voltage-Gated Sodium Channel
- Oligonucleotides, Antisense/metabolism
- RNA, Messenger/metabolism
- Sodium Channels/genetics*
- Sodium Channels/metabolism
- Transcription Factors/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- 20339120 Full text @ Circ. Res.
Chopra, S.S., Stroud, D.M., Watanabe, H., Bennett, J.S., Burns, C.G., Wells, K.S., Yang, T., Zhong, T.P., and Roden, D.M. (2010) Voltage-Gated Sodium Channels Are Required for Heart Development in Zebrafish. Circulation research. 106(8):1342-1350.
Rationale: Voltage-gated sodium channels initiate action potentials in excitable tissues. Mice in which Scn5A (the predominant sodium channel gene in heart) has been knocked out die early in development with cardiac malformations by mechanisms which have yet to be determined. Objective: Here we addressed this question by investigating the role of cardiac sodium channels in zebrafish heart development. Methods and Results: Transcripts of the functionally-conserved Scn5a homologs scn5Laa and scn5Lab were detected in the gastrulating zebrafish embryo and subsequently in the embryonic myocardium. Antisense knockdown of either channel resulted in marked cardiac chamber dysmorphogenesis and perturbed looping. These abnormalities were associated with decreased expression of the myocardial precursor genes nkx2.5, gata4, and hand2 in anterior lateral mesoderm and significant deficits in the production of cardiomyocyte progenitors. These early defects did not appear to result from altered membrane electrophysiology, as prolonged pharmacological blockade of sodium current failed to phenocopy channel knockdown. Moreover, embryos grown in calcium channel blocker-containing medium had hearts that did not beat but developed normally. Conclusions: These findings identify a novel, and possibly nonelectrogenic, role for cardiac sodium channels in heart development.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes