PUBLICATION
Cell adhesion glycoprotein vitronectin during Xenopus laevis embryogenesis
- Authors
- Luque, M.E., Serrano, M.A., Honoré, S.M., Mónaco, M.E., and Sánchez, S.S.
- ID
- ZDB-PUB-100330-1
- Date
- 2010
- Source
- Gene expression patterns : GEP 10(4-5): 207-213 (Journal)
- Registered Authors
- Keywords
- Xenopus laevis, Vitronectin, Embryogenesis, Cell adhesion, Glycoprotein, Development, Extracellular matrix, Cloning, cDNA, Somatomedin B, Transcripts, Stage, Tadpole, Heart, Gut, Derivatives, Notochord, Liver, Pronephros
- MeSH Terms
-
- Xenopus laevis/embryology
- Xenopus laevis/metabolism*
- Vitronectin/chemistry
- Vitronectin/metabolism*
- DNA, Complementary
- Embryonic Development
- In Situ Hybridization
- Animals
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Reverse Transcriptase Polymerase Chain Reaction
- Microscopy, Fluorescence
- Sequence Homology, Amino Acid
- Amino Acid Sequence
- PubMed
- 20302971 Full text @ Gene Expr. Patterns
Citation
Luque, M.E., Serrano, M.A., Honoré, S.M., Mónaco, M.E., and Sánchez, S.S. (2010) Cell adhesion glycoprotein vitronectin during Xenopus laevis embryogenesis. Gene expression patterns : GEP. 10(4-5):207-213.
Abstract
Vitronectin (vn) is a cell-adhesive glycoprotein present in blood and extracellular matrix of all vertebrates. In the present study we reported the cDNA cloning of Xenopus laevisvitronectin and its spatial and temporal expression pattern during the embryonic development of this important model organism. The deduced amino acid sequence of Xenopus laevis vn showed 49%, 47% and 43% identity with human, chicken and zebrafish orthologs, respectively, whereas the comparison with Xenopus tropicalis vn presented 85% identity. The structural organization consisting of a somatomedin B domain and two hemopexin-like domains was similar to higher vertebrate vitronectins. The vn transcripts were detected from stage 28 onward. At tadpole stages, vn is expressed in heart, gut derivatives and in the notochord. The protein was detected in heart, liver, foregut, pronephros and notochord at stages 43 and 47 of Xenopus embryos. Our results suggest that vitronectin is developmentally regulated and could participate in embryo organogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping