PUBLICATION
Zebrafish embryos exposed to alcohol undergo abnormal development of motor neurons and muscle fibers
- Authors
- Sylvain, N.J., Brewster, D.L., and Ali, D.W.
- ID
- ZDB-PUB-100317-7
- Date
- 2010
- Source
- Neurotoxicology and teratology 32(4): 472-480 (Journal)
- Registered Authors
- Keywords
- Ethanol, Skeletal muscle, Fetal alcohol syndrome, CNS
- MeSH Terms
-
- Animals
- Dose-Response Relationship, Drug
- Embryo, Nonmammalian/drug effects*
- Embryo, Nonmammalian/embryology
- Embryonic Development/drug effects*
- Ethanol/toxicity*
- Larva/drug effects*
- Larva/growth & development
- Motor Neurons/drug effects*
- Muscle Fibers, Fast-Twitch/drug effects*
- Muscle Fibers, Slow-Twitch/drug effects*
- Swimming
- Zebrafish/embryology*
- PubMed
- 20211721 Full text @ Neurotoxicol. Teratol.
Citation
Sylvain, N.J., Brewster, D.L., and Ali, D.W. (2010) Zebrafish embryos exposed to alcohol undergo abnormal development of motor neurons and muscle fibers. Neurotoxicology and teratology. 32(4):472-480.
Abstract
Children exposed to alcohol in utero have significantly delayed gross and fine motor skills, as well as deficiencies in reflex development. The reasons that underlie the motor deficits caused by ethanol (EtOH) exposure remain to be fully elucidated. The present study was undertaken to investigate the effects of embryonic alcohol exposure (1.5%, 2% and 2.5% EtOH) on motor neuron and muscle fiber morphology in 3days post fertilization (dpf) larval zebrafish. EtOH treated fish exhibited morphological deformities and fewer bouts of swimming in response to touch, compared with untreated fish. Immunolabelling with anti-acetylated tubulin indicated that fish exposed to 2.5% EtOH had significantly higher rates of motor neuron axon defects. Immunolabelling of primary and secondary motor neurons, using znp-1 and zn-8, revealed that fish exposed to 2% and 2.5% EtOH exhibited significantly higher rates of primary and secondary motor neuron axon defects compared to controls. Examination of red and white muscle fibers revealed that fish exposed to EtOH had significantly smaller red and white muscle fibers compared with controls. These findings indicate that motor neuron and muscle fiber morphology is affected by early alcohol exposure in zebrafish embryos, and that this may be related to deficits in locomotion.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping