PUBLICATION

A novel Wilms Tumor 1 (WT1) target gene negatively regulates the WNT signaling pathway

Authors
Kim, M.S., Yoon, S.K., Bollig, F., Kitagaki, J., Hur, W., Whye, N.J., Wu, Y.P., Rivera, M.N., Park, J.Y., Kim, H.S., Malik, K., Bell, D.W., Englert, C., Perantoni, A.O., and Lee, S.B.
ID
ZDB-PUB-100317-18
Date
2010
Source
The Journal of biological chemistry   285(19): 14585-14593 (Journal)
Registered Authors
Bollig, Frank, Englert, Christoph
Keywords
Gene expression, Kidney, Transcription target genes, Tumor suppressor, Wnt pathway
MeSH Terms
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism
  • Animals
  • Axin Protein
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism*
  • Chromatin Immunoprecipitation
  • Down-Regulation
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Immunoblotting
  • Immunoglobulin G/immunology
  • Immunoprecipitation
  • Kidney/cytology
  • Kidney/metabolism
  • Luciferases/metabolism
  • Mice
  • NIH 3T3 Cells
  • Phosphoproteins/genetics
  • Phosphoproteins/metabolism
  • Promoter Regions, Genetic
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • RNA, Small Interfering/pharmacology
  • Rabbits
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction*
  • WT1 Proteins/genetics
  • WT1 Proteins/metabolism*
  • Wnt Proteins/genetics
  • Wnt Proteins/metabolism*
  • Zebrafish
  • beta Catenin/genetics
  • beta Catenin/metabolism*
PubMed
20220130 Full text @ J. Biol. Chem.
Abstract
Mammalian kidney development requires the functions of the Wilms tumor gene WT1 and the WNT/beta-catenin signaling pathway. Recent studies have shown that WT1 negatively regulates WNT/beta-catenin signaling but the molecular mechanisms by which WT1 inhibits WNT/beta-catenin signaling are not completely understood. In this report, we identified a gene, CXXC5 which we have renamed WID (WT1-induced Inhibitor of Dishevelled), as a novel WT1 transcriptional target that negatively regulates WNT/beta-catenin signaling. WT1 activates WID transcription through the upstream enhancer region. In the developing kidney, Wid and Wt1 are coexpressed in podocytes of maturing nephrons. Structure-function analysis demonstrated that WID interacts with Dishevelled via its C-terminal CXXC zinc finger and Dishevelled binding domains and potently inhibits WNT/beta-catenin signaling in vitro and in vivo. WID is evolutionarily conserved and ablation of wid in zebrafish embryos with antisense morpholino oligonucleotides perturbs embryonic kidney development. Taken together, our results demonstrate that the WT1 negatively regulates WNT/beta-catenin pathway via its target gene WID and further suggest a role for WID in nephrogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping