PUBLICATION
CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function
- Authors
- Beqqali, A., Monshouwer-Kloots, J., Monteiro, R., Welling, M., Bakkers, J., Ehler, E., Verkleij, A., Mummery, C., and Passier, R.
- ID
- ZDB-PUB-100317-14
- Date
- 2010
- Source
- Journal of Cell Science 123(Pt 7): 1141-1150 (Journal)
- Registered Authors
- Bakkers, Jeroen
- Keywords
- CHAP, Synpo2l, Z-disc, Heart development, Muscle, Sarcomere
- MeSH Terms
-
- Active Transport, Cell Nucleus
- Animals
- Animals, Genetically Modified
- COS Cells
- Cell Nucleus/metabolism*
- Chlorocebus aethiops
- Embryo, Mammalian
- Gene Knockdown Techniques
- Heart/embryology
- Heart/physiology
- Mice
- Microfilament Proteins/genetics
- Microfilament Proteins/metabolism*
- Muscle Development
- Muscle, Skeletal/embryology
- Muscle, Skeletal/physiology
- Muscle, Skeletal/ultrastructure
- Myocytes, Cardiac/metabolism*
- Myocytes, Cardiac/ultrastructure
- Rats
- Sarcomeres/metabolism*
- Sarcomeres/ultrastructure
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 20215401 Full text @ J. Cell Sci.
Citation
Beqqali, A., Monshouwer-Kloots, J., Monteiro, R., Welling, M., Bakkers, J., Ehler, E., Verkleij, A., Mummery, C., and Passier, R. (2010) CHAP is a newly identified Z-disc protein essential for heart and skeletal muscle function. Journal of Cell Science. 123(Pt 7):1141-1150.
Abstract
In recent years, the perception of Z-disc function has changed from a passive anchor for myofilaments that allows transmission of force, to a dynamic multicomplex structure, capable of sensing and transducing extracellular signals. Here, we describe a new Z-disc protein, which we named CHAP (cytoskeletal heart-enriched actin-associated protein, expressed in differentiating heart and skeletal muscle in vitro and in vivo. Interestingly, in addition to its sarcomeric localization, CHAP was also able to translocate to the nucleus. CHAP was associated with filamentous actin in the cytoplasm and the nucleus when expressed ectopically in vitro, but in rat neonatal cardiomyocytes, CHAP disrupted the subcellular localization of alpha-actinin, another Z-disc protein. More importantly, knockdown of CHAP in zebrafish resulted in aberrant cardiac and skeletal muscle development and function. These findings suggest that CHAP is a critical component of the sarcomere with an important role in muscle development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping