PUBLICATION
Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from Black-boned sheep (Ovis aries)
- Authors
- He, Y.D., Liu, D.D., Xi, D.M., Yang, L.Y., Tan, Y.W., Liu, Q., Mao, H.M., and Deng, W.D.
- ID
- ZDB-PUB-100309-13
- Date
- 2010
- Source
- Molekuliarnaia biologiia 44(1): 20-27 (Journal)
- Registered Authors
- Keywords
- Black-boned sheep, Rab2A, Rab3A, Rab7A, tissue expression analysis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Cattle
- Dogs
- Gene Expression Profiling
- Humans
- Mice
- Molecular Sequence Data
- Phylogeny
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Rabbits
- Rats
- Sheep, Domestic/genetics*
- Tissue Distribution
- rab GTP-Binding Proteins/chemistry
- rab GTP-Binding Proteins/classification
- rab GTP-Binding Proteins/genetics*
- rab2 GTP-Binding Protein/chemistry
- rab2 GTP-Binding Protein/classification
- rab2 GTP-Binding Protein/genetics*
- rab3A GTP-Binding Protein/chemistry
- rab3A GTP-Binding Protein/classification
- rab3A GTP-Binding Protein/genetics*
- PubMed
- 20198855 Full text @ Mol. Biol. (Mosk.)
Citation
He, Y.D., Liu, D.D., Xi, D.M., Yang, L.Y., Tan, Y.W., Liu, Q., Mao, H.M., and Deng, W.D. (2010) Isolation, sequence identification and expression profile of three novel genes Rab2A, Rab3A and Rab7A from Black-boned sheep (Ovis aries). Molekuliarnaia biologiia. 44(1):20-27.
Abstract
Complete coding sequences of three Black-boned sheep (Ovis aries) genes Rab2A, Rab3A and Rab7A were amplified using reverse transcription polymerase chain reaction (RT-PCR) based on the conserved sequence information of cattle or other mammals known to be highly homologous to sheep ESTs. The Black-boned sheep Rab2A gene encodes a protein of 226 amino acids which contains the conserved putative RabL2 domain and is highly homologous to the Rab2A proteins of seven other species--cattle (96%), human (83%), Sumatran orangutan (82%), rat (81%), mouse (80%), African clawed frog (72%) and zebrafish (71%). The Black-boned sheep Rab3A gene encodes a protein of 220 amino acids that contains the conserved putative Rab3 domain and is very similar to the Rab3A proteins of four species--cattle (99%), African clawed frog (99%), Western clawed frog (98%) and zebrafish (95%). And the Black-boned sheep Rab7A gene encodes a protein of 207 amino acids that contains the conserved putative Rab7 domain and has high homology with the Rab7A proteins of six other species--human (99%), dog (99%), Sumatran orangutan (99%), zebrafish (97%), rabbit (97%) and African clawed frog (96%). Analysis of the phylogenetic tree has demonstrated that the Black-boned sheep Rab2A, Rab3A and Rab7A proteins share a common ancestor and the tissue expression analysis has shown that the corresponding genes are expressed in a range of tissues including leg muscle, kidney, skin, longissimus dorsi muscle, spleen, heart and liver. Our experiment is the first to provide the primary foundation for a further insight into these three sheep genes.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping