PUBLICATION

A role for the lysosomal protease cathepsin B in zebrafish follicular apoptosis

Authors
Eykelbosh, A.J., and Van Der Kraak, G.
ID
ZDB-PUB-100223-51
Date
2010
Source
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology   156(2): 218-223 (Journal)
Registered Authors
Van Der Kraak, Glen
Keywords
zebrafish reproduction, ovary, caspase-3, cathepsin B, atresia, apoptosis
MeSH Terms
  • Animals
  • Apoptosis*/drug effects
  • Caspase 3/metabolism
  • Cathepsin B/antagonists & inhibitors*
  • Cathepsin B/genetics
  • Cathepsin B/metabolism
  • Cell Survival/drug effects
  • Chorionic Gonadotropin/pharmacology
  • Cloning, Molecular
  • Culture Media, Serum-Free
  • Dipeptides/pharmacology
  • Estradiol/pharmacology
  • Female
  • Gene Expression Regulation, Enzymologic/drug effects
  • Humans
  • Lysosomes/drug effects
  • Lysosomes/enzymology*
  • Ovarian Follicle/cytology*
  • Ovarian Follicle/drug effects
  • Ovarian Follicle/enzymology*
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
20170740 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Abstract
This study presents evidence that cathepsin B, a lysosomal protease, may be involved in the regulation of apoptosis during serum-starvation in teleost follicles. Zebrafish vitellogenic follicles were isolated, incubated under serum-free conditions and homogenized. The follicle extracts demonstrated caspase-3-like activity using the fluorogenic substrate DEVD-AMC, indicating the onset of apoptosis. Cathepsin B activity as measured using the fluorogenic cathepsin B substrate, Z-Arg-Arg-AMC was elevated within the first 6h of incubation in serum-free media and coincided with the onset of apoptosis. This increase in cathepsin B activity was sensitive to the cathepsin B inhibitor, CA-074-ME. Furthermore, adding CA-074-ME to the follicle incubation blocked caspase-3-like activation, suggesting that cathepsin B activity is a positive regulator of the apoptotic cascade during serum-starvation. Interestingly, the increase in cathepsin-B-like activity was not preceded by an increase in cathepsin B mRNA transcription, suggesting that regulation of this enzyme is at a level other than of the gene. These results suggest a regulatory role for cathepsin B during follicular apoptosis in zebrafish ovarian follicles.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping