PUBLICATION
A role for the lysosomal protease cathepsin B in zebrafish follicular apoptosis
- Authors
- Eykelbosh, A.J., and Van Der Kraak, G.
- ID
- ZDB-PUB-100223-51
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 156(2): 218-223 (Journal)
- Registered Authors
- Van Der Kraak, Glen
- Keywords
- zebrafish reproduction, ovary, caspase-3, cathepsin B, atresia, apoptosis
- MeSH Terms
-
- Animals
- Apoptosis*/drug effects
- Caspase 3/metabolism
- Cathepsin B/antagonists & inhibitors*
- Cathepsin B/genetics
- Cathepsin B/metabolism
- Cell Survival/drug effects
- Chorionic Gonadotropin/pharmacology
- Cloning, Molecular
- Culture Media, Serum-Free
- Dipeptides/pharmacology
- Estradiol/pharmacology
- Female
- Gene Expression Regulation, Enzymologic/drug effects
- Humans
- Lysosomes/drug effects
- Lysosomes/enzymology*
- Ovarian Follicle/cytology*
- Ovarian Follicle/drug effects
- Ovarian Follicle/enzymology*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 20170740 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Eykelbosh, A.J., and Van Der Kraak, G. (2010) A role for the lysosomal protease cathepsin B in zebrafish follicular apoptosis. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 156(2):218-223.
Abstract
This study presents evidence that cathepsin B, a lysosomal protease, may be involved in the regulation of apoptosis during serum-starvation in teleost follicles. Zebrafish vitellogenic follicles were isolated, incubated under serum-free conditions and homogenized. The follicle extracts demonstrated caspase-3-like activity using the fluorogenic substrate DEVD-AMC, indicating the onset of apoptosis. Cathepsin B activity as measured using the fluorogenic cathepsin B substrate, Z-Arg-Arg-AMC was elevated within the first 6h of incubation in serum-free media and coincided with the onset of apoptosis. This increase in cathepsin B activity was sensitive to the cathepsin B inhibitor, CA-074-ME. Furthermore, adding CA-074-ME to the follicle incubation blocked caspase-3-like activation, suggesting that cathepsin B activity is a positive regulator of the apoptotic cascade during serum-starvation. Interestingly, the increase in cathepsin-B-like activity was not preceded by an increase in cathepsin B mRNA transcription, suggesting that regulation of this enzyme is at a level other than of the gene. These results suggest a regulatory role for cathepsin B during follicular apoptosis in zebrafish ovarian follicles.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping