PUBLICATION
Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development
- Authors
- Germanà, A., Laurà, R., Montalbano, G., Guerrera, M.C., Amato, V., Zichichi, R., Campo, S., Ciriaco, E., and Vega, J.A.
- ID
- ZDB-PUB-100223-44
- Date
- 2010
- Source
- Cellular and molecular neurobiology 30(5): 787-793 (Journal)
- Registered Authors
- Amato, Valentina, Germanà, Antonino, Guerrera, Maria Cristina, Montalbano, Giuseppe, Zichichi, Rosalia
- Keywords
- Lateral line system, Neuromast, TrkB, Brain-derived neurotrophic factor (BDNF), Zebrafish
- MeSH Terms
-
- Aging/genetics
- Animals
- Animals, Genetically Modified
- Blotting, Western
- Brain-Derived Neurotrophic Factor/genetics*
- Brain-Derived Neurotrophic Factor/metabolism
- Fluorescence
- Gene Expression Regulation, Developmental*
- Immunohistochemistry
- Lateral Line System/cytology
- Lateral Line System/metabolism*
- Lateral Line System/ultrastructure
- Protein Transport
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Receptor, trkB/genetics*
- Receptor, trkB/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics*
- PubMed
- 20162349 Full text @ Cell. Mol. Neurobiol.
Citation
Germanà, A., Laurà, R., Montalbano, G., Guerrera, M.C., Amato, V., Zichichi, R., Campo, S., Ciriaco, E., and Vega, J.A. (2010) Expression of Brain-Derived Neurotrophic Factor and TrkB in the Lateral Line System of Zebrafish During Development. Cellular and molecular neurobiology. 30(5):787-793.
Abstract
The neuromasts of the lateral line system are regarded as a model to study the mechanisms of hearing, deafness, and ototoxicity. The neurotrophins (NTs), especially brain-derived neurotrophic factor (BDNF), and its signaling receptor TrkB are involved in the development and maintenance of neuromasts. To know the period in which the BDNF/TrkB complex has more effects in the neuromast biology, the age-related changes were studied. Normal zebrafish from 10 to 180 days post-fertilization (dpf), as well as transgenic ET4 zebrafish 10 and 20 dpf, was analyzed using qRT-PCR, western blot, and immunohistochemistry. BDNF and TrkB mRNAs followed a parallel course, peaking at 20 dpf, and thereafter progressively decreased. Specific immunoreactivity for BDNF and TrkB was found co-localized in all hairy cells of neuromasts in 20 and 30 dpf; then, the number of immunoreactive cells decreased, and by 180 dpf BDNF remains restricted to a subpopulation of hairy cells, and TrkB to a few number of sensory and non-sensory cells. At all ages examined, TrkB immunoreactivity was detected in sensory ganglia innervating the neuromasts. The present results demonstrate that there is a parallel time-related decline in the expression of BDNF and TrkB in zebrafish. Also, the patterns of cell expression suggest that autocrine/paracrine mechanisms for this NT system might occur within the neuromasts. Because TrkB in lateral line ganglia did not vary with age, their neurons are potentially capable to respond to BDNF during the entire lifespan of zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping