PUBLICATION

Development of multilineage adult hematopoiesis in the zebrafish with a runx1 truncation mutation

Authors
Sood, R., English, M.A., Belele, C.L., Jin, H., Bishop, K., Haskins, R., McKinney, M.C., Chahal, J., Weinstein, B.M., Wen, Z., and Liu, P.P.
ID
ZDB-PUB-100223-24
Date
2010
Source
Blood   115(14): 2806-2809 (Journal)
Registered Authors
English, Milton A., Haskins, Rebecca, Liu, Pu Paul, McKinney, Cathy, Sood, Raman, Weinstein, Brant M., Wen, Zilong
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Core Binding Factor Alpha 2 Subunit/genetics
  • Core Binding Factor Alpha 2 Subunit/metabolism*
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Hematopoiesis/physiology*
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/metabolism*
  • Mesonephros/cytology
  • Mesonephros/embryology
  • Mesonephros/metabolism
  • Mutation*
  • Platelet Membrane Glycoprotein IIb/biosynthesis
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
20154212 Full text @ Blood
Abstract
Runx1 is required for the emergence of hematopoietic stem cells (HSCs) from hemogenic endothelium during embryogenesis. However, its role in the generation and maintenance of HSCs during adult hematopoiesis remains uncertain. Here, we present analysis of a zebrafish mutant line carrying a truncation mutation, W84X, in runx1. The runx1(W84X/W84X) embryos showed blockage in the initiation of definitive hematopoiesis, but some embryos were able to recover from a larval "bloodless" phase and develop to fertile adults with multi-lineage hematopoiesis. Using cd41-GFP transgenic zebrafish and lineage tracing, we demonstrated that the runx1(W84X/W84X) embryos developed cd41(+) HSCs in the aorta-gonad-mesonephros (AGM) region, which later migrated to the kidney, the site of adult hematopoiesis. Overall, our data suggest that in zebrafish adult HSCs can be formed without an intact runx1.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes