PUBLICATION
Temporal dynamics of myelination in the zebrafish spinal cord
- Authors
- Buckley, C.E., Marguerie, A., Alderton, W.K., and Franklin, R.J.
- ID
- ZDB-PUB-100211-25
- Date
- 2010
- Source
- Glia 58(7): 802-812 (Journal)
- Registered Authors
- Keywords
- oligodendrocyte, screening, model, therapy, multiple sclerosis
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Body Patterning/physiology
- Cell Differentiation/physiology*
- Gene Expression Regulation, Developmental/physiology
- Green Fluorescent Proteins
- Growth Cones/metabolism*
- Growth Cones/ultrastructure
- Immunohistochemistry
- Microscopy, Electron, Transmission
- Models, Animal
- Myelin Basic Protein/genetics
- Myelin Basic Protein/metabolism
- Nerve Fibers, Myelinated/metabolism*
- Nerve Fibers, Myelinated/ultrastructure
- Neurogenesis/physiology
- Oligodendroglia/cytology
- Oligodendroglia/metabolism
- RNA, Messenger/metabolism
- Spinal Cord/cytology
- Spinal Cord/embryology*
- Spinal Cord/metabolism*
- Time Factors
- Zebrafish/embryology*
- PubMed
- 20140960 Full text @ Glia
Citation
Buckley, C.E., Marguerie, A., Alderton, W.K., and Franklin, R.J. (2010) Temporal dynamics of myelination in the zebrafish spinal cord. Glia. 58(7):802-812.
Abstract
Knowledge of the precise timing of myelination is critical to the success of zebrafish-based in vivo screening strategies for potential remyelination therapies. This study provides a systematic review of the timing of myelination in the zebrafish spinal cord and a critique of techniques by which it may be accurately assessed. The onset of myelination was found to be 3 days postfertilization (d.p.f.); earlier than previously reported. This coincided with the dorsal migration and differentiation of oligodendrocytes and the expression of myelin basic protein (Mbp) transcripts and protein. Our data suggests that immunohistochemistry with zebrafish-specific anti-Mbp from 3 d.p.f. is the optimal histological method for myelin visualization, while quantification of myelination is more reliably achieved by quantitative polymerase chain reaction (qPCR) for mbp from 5 d.p.f.. Transgenic fluorescent lines such as olig2:EGFP can be used to assess oligodendrocyte cell number at 3 d.p.f. and the development of new, more specific lines may enable real time visualization of myelin itself. Quantitative ultrastructural analysis revealed that the myelination of zebrafish axons is regulated according to axonal growth and not absolute axonal size. This study confirms the use of the zebrafish larvae as a versatile and efficient in vivo model of myelination and provides a platform on which future myelination screening studies can be based.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping