PUBLICATION
Distinct developmental expression of two elongase family members in zebrafish
- Authors
- Tan, S.H., Chung, H.H., and Shu-Chien, A.C.
- ID
- ZDB-PUB-100211-19
- Date
- 2010
- Source
- Biochemical and Biophysical Research Communications 393(3): 397-403 (Journal)
- Registered Authors
- Keywords
- elovl2, elovl5, liver, intestine, brain, pronephros, zebrafish
- MeSH Terms
-
- Acetyltransferases/genetics*
- Acetyltransferases/metabolism
- Animals
- Embryonic Development/genetics
- Fatty Acid Desaturases/genetics*
- Fatty Acids, Unsaturated/metabolism*
- Gene Expression Regulation, Developmental*
- Gene Expression Regulation, Enzymologic*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 20138842 Full text @ Biochem. Biophys. Res. Commun.
Citation
Tan, S.H., Chung, H.H., and Shu-Chien, A.C. (2010) Distinct developmental expression of two elongase family members in zebrafish. Biochemical and Biophysical Research Communications. 393(3):397-403.
Abstract
Despite the known importance of long chained polyunsaturated fatty acids (LC-PUFA) during development, very little is known about their utilization and biosynthesis during embryogenesis. Combining the advantages of the existence of a complete range of enzymes required for LC-PUFA biosynthesis and the well established developmental biology tools in zebrafish, we examined the expression patterns of three LC-PUFA biosynthesis genes, Elovl2-like elongase (elovl2), Elovl5-like elongase (elovl5) and fatty acyl desaturase (fad) in different zebrafish developmental stages. The presence of all 3 genes in the brain as early as 24 hours post fertilization (hpf) implies LC-PUFA synthesis activity in the embryonic brain. This expression eventually subsides from 72hpf onwards, coinciding with the initiation of elovl2 and fad expression in the liver and intestine, 2 organs known to be involved in adult fish LC-PUFA biosynthesis. Collectively, these patterns strongly suggest the necessity for localized production of LC-PUFA in the brain during in early stage embryos prior to the maturation of the liver and intestine. Interestingly, we also showed a specific expression of elovl5 in the proximal convoluted tubule (PCT) of the zebrafish pronephros, suggesting a possible new role for LC-PUFA in kidney development and function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping