PUBLICATION
Localization of c-mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba
- Authors
- Suzuki, H., Tsukahara, T., and Inoue, K.
- ID
- ZDB-PUB-100202-5
- Date
- 2009
- Source
- Bioscience trends 3(3): 96-104 (Journal)
- Registered Authors
- Inoue, Kunio
- Keywords
- CPEB, c-mos, CELF/Bruno, RNA localization, oocyte maturation
- MeSH Terms
-
- Animals
- Blotting, Northern
- Cell Polarity/physiology
- Cloning, Molecular
- DNA Primers/genetics
- DNA, Complementary/genetics
- Genes, mos/genetics*
- Immunoprecipitation
- In Situ Hybridization
- Oocytes/cytology
- Oocytes/metabolism*
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- RNA-Binding Proteins/metabolism*
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism*
- PubMed
- 20103830
Citation
Suzuki, H., Tsukahara, T., and Inoue, K. (2009) Localization of c-mos mRNA around the animal pole in the zebrafish oocyte with Zor-1/Zorba. Bioscience trends. 3(3):96-104.
Abstract
In oocytes, many maternally supplied products are stored, and these products play important roles in cell cycle regulation and early development. Mos protein, which is coded on the c-mos gene, promotes oocyte maturation and is involved in MAP-kinase signaling pathway. In Xenopus, maternally supplied c-mos mRNA undergoes poly(A) addition, and translational activation via CPE (cytoplasmic polyadenylation element) and CPEB (CPE binding protein). The elongated poly(A) is shortened and the c-mos mRNA is degraded during early embryogenesis via EDEN (embryo deadenylation element) and EDEN-BP (EDEN-binding protein). We cloned the full-length zebrafish c-mos gene, which is conserved at the protein coding region in vertebrates. c-mos mRNA has two putative CPE sequences in its 3'UTR, which binds to zebrafish CPEB homologous protein, Zor-1. We could not observe EDEN sequence, and could not detect interaction between c-mos mRNA and zebrafish EDEN-BP homologous protein, Brul, even though immuno precipitation and RT-PCR experiments suggested that c-mos mRNA interacts with Zor-1 in vivo. Interestingly, we found c-mos mRNA is located in the animal cortex of zebrafish oocyte, where Zor-1 protein exists. Taken together, these results suggest that the animal cortex is the central core of oocyte maturation in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping