PUBLICATION
A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer
- Authors
- Chen, Y., Zhao, M., Wang, S., Chen, J., Wang, Y., Cao, Q., Zhou, W., Liu, J., Xu, Z., Tong, G., and Li, J.
- ID
- ZDB-PUB-100128-3
- Date
- 2009
- Source
- Journal of Cancer Research and Clinical Oncology 135(9): 1265-1276 (Journal)
- Registered Authors
- Keywords
- DYX1C1, Heat shock protein, Tetratricopeptide repeats, Protein interaction, Breast cancer
- MeSH Terms
-
- Nuclear Proteins/genetics
- Nuclear Proteins/metabolism*
- Mutagenesis, Site-Directed
- Reverse Transcriptase Polymerase Chain Reaction
- RNA, Messenger/genetics
- Humans
- HSP70 Heat-Shock Proteins/metabolism*
- Up-Regulation/genetics
- HSP90 Heat-Shock Proteins/metabolism*
- Female
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Two-Hybrid System Techniques
- Breast Neoplasms/genetics
- Breast Neoplasms/metabolism*
- Breast Neoplasms/pathology
- Molecular Sequence Data
- Amino Acid Sequence
- PubMed
- 19277710 Full text @ J. Cancer Res. Clin. Oncol.
Citation
Chen, Y., Zhao, M., Wang, S., Chen, J., Wang, Y., Cao, Q., Zhou, W., Liu, J., Xu, Z., Tong, G., and Li, J. (2009) A novel role for DYX1C1, a chaperone protein for both Hsp70 and Hsp90, in breast cancer. Journal of Cancer Research and Clinical Oncology. 135(9):1265-1276.
Abstract
AIMS: With three consecutive tetratricopeptide repeat (TPR) motifs at its C-terminus essential for neuronal migration, and a p23 domain at its N-terminus, DYX1C1 was the first gene proposed to have a role in developmental dyslexia. In this study, we attempted to identify the potential interaction of DYX1C1 and heat shock protein, and the role of DYX1C1 in breast cancer. MAIN METHODS: GST pull-down, a yeast two-hybrid system, RT-PCR, site-directed mutagenesis approach. KEY FINDINGS: Our study initially confirmed DYX1C1, a dyslexia related protein, could interact with Hsp70 and Hsp90 via GST pull-down and a yeast two-hybrid system. And we verified that EEVD, the C-terminal residues of DYX1C1, is responsible for the identified association. Further, DYX1C1 mRNA was significantly overexpressed in malignant breast tumor, linking with the up-regulated expression of Hsp70 and Hsp90. SIGNIFICANCE: These results suggest that DYX1C1 is a novel Hsp70 and Hsp90-interacting co-chaperone protein and its expression is associated with malignancy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping