PUBLICATION
Cloning and expression of P450c17-I (17alpha-hydroxylase/17,20-lyase) in brain and ovary during gonad development in Cynoglossus semilaevis
- Authors
- Chen, C.F., Wen, H.S., Wang, Z.P., He, F., Zhang, J.R., Chen, X.Y., Jin, G.X., Shi, B., Shi, D., Yang, Y.P., Li, J.F., Qi, B.X., and Li, N.
- ID
- ZDB-PUB-100119-4
- Date
- 2010
- Source
- Fish physiology and biochemistry 36(4): 1001-1012 (Journal)
- Registered Authors
- Keywords
- Half-smooth tongue sole, P450c17, mRNA expression, Physiological function, Testosterone, Estradiol-17β
- MeSH Terms
-
- Cloning, Molecular
- Flatfishes/genetics*
- DNA Primers/genetics
- Molecular Sequence Data
- Radioimmunoassay
- Ovary/enzymology*
- Ovary/growth & development
- Female
- Steroid 17-alpha-Hydroxylase/genetics*
- Steroid 17-alpha-Hydroxylase/metabolism*
- Sequence Alignment
- Sequence Homology
- Testosterone/blood
- Animals
- Brain/enzymology*
- Histological Techniques
- Species Specificity
- Amino Acid Sequence
- Gene Expression Profiling
- Analysis of Variance
- Reverse Transcriptase Polymerase Chain Reaction
- Estradiol/blood
- DNA, Complementary/genetics
- PubMed
- 20069358 Full text @ Fish Physiol. Biochem.
Citation
Chen, C.F., Wen, H.S., Wang, Z.P., He, F., Zhang, J.R., Chen, X.Y., Jin, G.X., Shi, B., Shi, D., Yang, Y.P., Li, J.F., Qi, B.X., and Li, N. (2010) Cloning and expression of P450c17-I (17alpha-hydroxylase/17,20-lyase) in brain and ovary during gonad development in Cynoglossus semilaevis. Fish physiology and biochemistry. 36(4):1001-1012.
Abstract
Cytochrome P450c17 (CYP17, 17a-hydroxylase/17,20-lyase) is a critical enzyme in the production of androgens and estrogens in vertebrates. A 2,469 bp full length cDNA of P450c17-I (CYP17A1) has been isolated from the ovary of half-smooth tongue sole, Cynoglossus semilaevis which encodes 509 amino acids. Additionally, a relatively shorter cDNA (1,742 bp), a likely result of polyadenylation, was also found. The putative P450c17-I enzyme shares high sequence identity with that of the fathead minnow (73%), zebrafish (71%), the Japanese eel (70%), catfish (70%), tilapia (79%), three-spined stickleback (81%), medaka (79%), dogfish (60%), chicken (65%), rat (47%), and human (49%). Semi-quantitative RT-PCR analysis of spatial expression showed the enzyme was predominantly expressed in the ovaries and the brain. P450c17-I was also detected in the stomach, intestine, gill, spleen, kidney, and head kidney, albeit weakly. Further examination of temporal expression pattern of P450c17-I in ovary and brain revealed developmental stage-dependency. In addition to this our data on T and E2 levels further endorse the critical role of P450c17-I during shift in steroidogenesis. Based on the present study we indicate an important role for P450c17-I during ovarian development. However, further studies are needed at transcriptional regulation level for deeper insights into the physiological functions of P450c17-I.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping