PUBLICATION

Growth and transcriptional effect of dietary 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio)

Authors
Chen, T.H., Cheng, Y.M., Cheng, J.O., Chou, C.T., Hsiao, Y.C., and Ko, F.C.
ID
ZDB-PUB-100119-10
Date
2010
Source
Ecotoxicology and environmental safety   73(3): 377-383 (Journal)
Registered Authors
Keywords
Polybrominated diphenyl ethers, Brominated flame retardant, Zebrafish, Gene expression
MeSH Terms
  • Male
  • Animal Feed
  • Halogenated Diphenyl Ethers/metabolism
  • Halogenated Diphenyl Ethers/toxicity*
  • Female
  • Life Cycle Stages/drug effects*
  • Life Cycle Stages/physiology
  • Gene Expression Profiling
  • Animals
  • Cytochrome P-450 CYP1A1/genetics
  • Cytochrome P-450 CYP1A1/metabolism
  • Zebrafish/physiology*
  • Body Size/drug effects
  • Toxicity Tests
  • Longevity/drug effects
  • RNA, Messenger/metabolism
  • Water Pollutants, Chemical/metabolism
  • Water Pollutants, Chemical/toxicity*
  • Transcription, Genetic/drug effects*
  • Gene Expression Regulation, Enzymologic/drug effects
(all 20)
PubMed
20074802 Full text @ Ecotoxicol. Environ. Saf.
Abstract
In this study, zebrafish (Danio rerio) were fed food dosed with pure PBDE-47 (2,2',4,4'-tetrabromodiphenyl ether) congener or a blank from 20 to 60 day post-hatch (dph). At 38dph, half of the fish were sampled for body size measurements and gene expression analyzes (CYP1A1, VTG, TTR, D1, and TSH-beta). At 60dph, body size was measured again for all fish remaining. Whole-fish histology was performed and the PBDE levels in fish were determined. PBDE treated fish was significantly smaller at 38dph but not at 60dph. No apparent histopathological effect was observed. In the PBDE treated fish, there was a weak induction of CYP1A1 mRNA transcription, but not of the other genes. The tissue levels of PBDE-47 were comparable to that found in other wild fish reported in the literature, indicating that our exposure level was ecologically relevant.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Chen, T.H., Cheng, Y.M., Cheng, J.O., Chou, C.T., Hsiao, Y.C., and Ko, F.C. (2010) Growth and transcriptional effect of dietary 2,2',4,4'-tetrabromodiphenyl ether (PBDE-47) exposure in developing zebrafish (Danio rerio). Ecotoxicology and environmental safety. 73(3):377-383.