PUBLICATION
The zebrafish homeobox gene irxl1 is required for brain and pharyngeal arch morphogenesis
- Authors
- Chuang, H.N., Cheng, H.Y., Hsiao, K.M., Lin, C.W., Lin, M.L., and Pan, H.
- ID
- ZDB-PUB-091221-25
- Date
- 2010
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 239(2): 639-650 (Journal)
- Registered Authors
- Pan, Huichin
- Keywords
- cartilage, homeobox, Iroquois, pharyngeal arches, zebrafish
- MeSH Terms
-
- Gene Knockdown Techniques
- Cloning, Molecular
- Injections
- Gene Expression Profiling
- Cell Movement
- Molecular Sequence Data
- Brain/embryology*
- Branchial Region/embryology*
- Zebrafish
- Embryonic Development*
- Genes, Homeobox/genetics*
- Neural Crest/cytology
- Cell Differentiation
- Chondrocytes/cytology
- Fish Proteins/genetics
- Amino Acid Sequence
- RNA, Complementary*
- Animals
- Homeodomain Proteins/genetics*
- PubMed
- 20014404 Full text @ Dev. Dyn.
Citation
Chuang, H.N., Cheng, H.Y., Hsiao, K.M., Lin, C.W., Lin, M.L., and Pan, H. (2010) The zebrafish homeobox gene irxl1 is required for brain and pharyngeal arch morphogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 239(2):639-650.
Abstract
Iroquois homeobox-like 1 (irxl1) is a novel member of the TALE superfamily of homeobox genes that is most closely related to the Iroquois class. We have identified the zebrafish irxl1 gene and characterized its structure. The protein contains a homeodomain that shares 100% sequence identity with other vertebrate orthologs. During embryogenesis, irxl1 is expressed from 18 hours postfertilization onward and prominent expression is detected in the pharyngeal arches. Knockdown of irxl1 by morpholinos results in malformed brain and arch structures, which can be partially rescued by cRNA injection. The heads of the morphants become small and flat, and extensions along the anterior-posterior/dorso-ventral axes are reduced without affecting regional specification. Loss of irxl1 function also causes deficit in neural crest cells which consequently results in partial loss of craniofacial muscles and severe deformation of arch cartilages. These observations suggest that irxl1 may regulate factors involved in brain and pharyngeal arch development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping