PUBLICATION
Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin
- Authors
- Ohta, K., Lupo, G., Kuriyama, S., Keynes, R., Holt, C.E., Harris, W.A., Tanaka, H., and Ohnuma, S.
- ID
- ZDB-PUB-091204-9
- Date
- 2004
- Source
- Developmental Cell 7(3): 347-358 (Journal)
- Registered Authors
- Harris, William A., Tanaka, Hideaki
- Keywords
- none
- MeSH Terms
-
- Lens, Crystalline/embryology
- Gene Library
- RNA, Small Interfering/metabolism
- Molecular Sequence Data
- Xenopus Proteins
- Bone Morphogenetic Protein 4
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- Gastrula/cytology
- Gene Expression Regulation, Developmental*
- Cloning, Molecular
- Protein Binding
- Xenopus
- In Situ Hybridization
- Bone Morphogenetic Proteins/metabolism*
- Intercellular Signaling Peptides and Proteins/metabolism*
- Amino Acid Sequence
- Neurons/metabolism
- Proteoglycans/physiology*
- Glycoproteins/metabolism*
- Blotting, Western
- Animals
- Chick Embryo
- PubMed
- 15363410 Full text @ Dev. Cell
Citation
Ohta, K., Lupo, G., Kuriyama, S., Keynes, R., Holt, C.E., Harris, W.A., Tanaka, H., and Ohnuma, S. (2004) Tsukushi functions as an organizer inducer by inhibition of BMP activity in cooperation with chordin. Developmental Cell. 7(3):347-358.
Abstract
During chick gastrulation, inhibition of BMP signaling is required for primitive streak formation and induction of Hensen's node. We have identified a unique secreted protein, Tsukushi (TSK), which belongs to the Small Leucine-Rich Proteoglycan (SLRP) family and is expressed in the primitive streak and Hensen's node. Grafts of cells expressing TSK in combination with the middle primitive streak induce an ectopic Hensen's node, while electroporation of TSK siRNA inhibits induction of the node. In Xenopus embryos, TSK can block BMP function and induce a secondary dorsal axis, while it can dorsalize ventral mesoderm and induce neural tissue in embryonic explants. Biochemical analysis shows that TSK binds directly to both BMP and chordin and forms a ternary complex with them. These observations indicate that TSK is an essential dorsalizing factor involved in the induction of Hensen's node.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping