PUBLICATION
Characterisation and differential expression during development of a duplicate Disabled-1 (Dab1) gene from zebrafish
- Authors
- Herrero-Turrión, M.J., Velasco, A., Arevalo, R., Aijón, J., and Lara, J.M.
- ID
- ZDB-PUB-091204-21
- Date
- 2010
- Source
- Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology 155(3): 217-229 (Journal)
- Registered Authors
- Keywords
- Alternative splicing, Disabled 1 (Dab1), Duplication, Gene expression, In silico, Phylogeny, Teleosts, Zebrafish (Danio rerio)
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics*
- Amino Acid Sequence
- Animals
- Base Sequence
- Computational Biology
- Conserved Sequence
- Evolution, Molecular
- Female
- Gene Expression Regulation, Developmental*
- Genes, Duplicate/genetics*
- Genomics
- Humans
- Male
- Mice
- Molecular Sequence Data
- Protein Isoforms/chemistry
- Protein Isoforms/genetics
- Protein Isoforms/metabolism
- Rats
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/genetics*
- Zebrafish/growth & development*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 19931637 Full text @ Comp. Biochem. Physiol. B Biochem. Mol. Biol.
Citation
Herrero-Turrión, M.J., Velasco, A., Arevalo, R., Aijón, J., and Lara, J.M. (2010) Characterisation and differential expression during development of a duplicate Disabled-1 (Dab1) gene from zebrafish. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 155(3):217-229.
Abstract
We identified a new duplicated Dab1 gene (drDab1b) spanning around 25kb of genomic DNA in zebrafish. Located in zebrafish chromosome 2, it is composed of 11 encoding exons and shows high sequence similarity to other Dab1 genes, including drDab1a, a zebrafish Dab1 gene previously characterised. drDab1b encodes by alternative splicing at least five different isoforms. Both drDab1a and drDab1b show differential gene expression levels in distinct adult tissues and during development. drDab1b is expressed in peripheral tissues (gills, heart, intestine, muscle), the immune system (blood, liver) and the central nervous system (CNS), whereas drDab1a is only expressed in gills, muscle and the CNS, suggesting a division of functions for two Dab1 genes in zebrafish adult tissues. RT-PCR analysis also reveals that both drDab1 genes show distinct developmental-specific expression patterns throughout development. drDab1b expression was higher than that of drDab1a, suggesting a major role of drDab1b in comparison with drDab1a during development and in different adult tissues. In addition, new putative Dab1 (a and/or b) from different teleost species were identified in silico and predicted protein products are compared with the previously characterised Dab1, demonstrating that the Dab1b group is more ancestral than their paralogue, the Dab1a group.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping