PUBLICATION
A non-coding antisense RNA in tie-1 locus regulates tie-1 function in vivo
- Authors
- Li, K., Blum, Y., Verma, A., Liu, Z., Pramanik, K., Leigh, N.R., Chun, C.Z., Samant, G.V., Zhao, B., Garnaas, M.K., Horswill, M.A., Stanhope, S.A., North, P.E., Miao, R.Q., Wilkinson, G.A., Affolter, M., and Ramchandran, R.
- ID
- ZDB-PUB-091120-8
- Date
- 2010
- Source
- Blood 115(1): 133-139 (Journal)
- Registered Authors
- Affolter, Markus, Chun, Chang Zoon, Garnaas, Maija, Horswill, Mark, Leigh, Noah, Li, Keguo, Ramchandran, Ramani, Samant, Ganesh, Verma, Anjali
- Keywords
- none
- MeSH Terms
-
- Neovascularization, Physiologic/drug effects
- Vascular Diseases/genetics
- Vascular Diseases/pathology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Endothelial Cells/drug effects
- Endothelial Cells/metabolism
- Endothelial Cells/pathology
- Phenotype
- Humans
- Species Specificity
- RNA, Untranslated/genetics*
- RNA, Untranslated/metabolism
- Mice
- Animals
- Gene Expression Regulation/drug effects
- Intercellular Junctions/drug effects
- Intercellular Junctions/metabolism
- Intercellular Junctions/pathology
- Vascular Endothelial Growth Factor A/pharmacology
- Endothelium/drug effects
- Endothelium/metabolism
- Endothelium/pathology
- Gene Expression Profiling
- Genetic Loci/genetics*
- Zebrafish/genetics*
- RNA, Antisense/genetics*
- RNA, Antisense/metabolism
- Receptor, TIE-1/genetics
- Receptor, TIE-1/metabolism
- PubMed
- 19880500 Full text @ Blood
Citation
Li, K., Blum, Y., Verma, A., Liu, Z., Pramanik, K., Leigh, N.R., Chun, C.Z., Samant, G.V., Zhao, B., Garnaas, M.K., Horswill, M.A., Stanhope, S.A., North, P.E., Miao, R.Q., Wilkinson, G.A., Affolter, M., and Ramchandran, R. (2010) A non-coding antisense RNA in tie-1 locus regulates tie-1 function in vivo. Blood. 115(1):133-139.
Abstract
Recently, messenger RNAs in eukaryotes have shown to associate with antisense (AS) transcript partners that are often referred to as long noncoding RNAs (lncRNAs) whose function is largely unknown. Here, we have identified a natural AS transcript for tyrosine kinase containing immunoglobulin and epidermal growth factor homology domain-1 (tie-1), tie-1AS lncRNA in zebrafish, mouse and humans. In embryonic zebrafish, tie-1AS lncRNA transcript is expressed temporally and spatially in vivo with its native target, the tie-1 coding transcript and in additional locations (ear & brain). The tie-1AS lncRNA selectively binds tie-1 mRNA in vivo and regulates tie-1 transcript levels resulting in specific defects in endothelial cell contact junctions in vivo and in vitro. The ratio of tie-1 vs. tie-1AS lncRNA is altered in human vascular anomaly samples. These results directly implicate non-coding RNA mediated transcriptional regulation of gene expression as a fundamental control mechanism for physiological process such as vascular development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping