PUBLICATION
Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol
- Authors
- Liu, C., Deng, J., Yu, L., Ramesh, M., and Zhou, B.
- ID
- ZDB-PUB-091120-5
- Date
- 2010
- Source
- Aquatic toxicology (Amsterdam, Netherlands) 96(1): 70-76 (Journal)
- Registered Authors
- Yu, Liqun
- Keywords
- Fluorotelomer alcohols, Fecundity, Gamatogenesis, Sex hormone, Gene transcription, Reproductive toxicity
- MeSH Terms
-
- Animals
- Endocrine Disruptors/toxicity*
- Estradiol/metabolism
- Female
- Fertility/drug effects
- Follicle Stimulating Hormone, beta Subunit/metabolism
- Gene Expression Regulation/drug effects
- Hydrocarbons, Fluorinated/toxicity*
- Luteinizing Hormone/metabolism
- Male
- Oocytes/drug effects
- Pituitary Gland/drug effects
- Reproduction/drug effects*
- Sperm Maturation/drug effects
- Testosterone/metabolism
- Water Pollutants, Chemical/toxicity*
- Zebrafish/metabolism
- Zebrafish/physiology*
- Zebrafish Proteins/metabolism
- PubMed
- 19879662 Full text @ Aquat. Toxicol.
Citation
Liu, C., Deng, J., Yu, L., Ramesh, M., and Zhou, B. (2010) Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol. Aquatic toxicology (Amsterdam, Netherlands). 96(1):70-76.
Abstract
The fluorotelomer alcohols (FTOHs) have been detected in the aquatic environment and shown potential estrogenic activity, but the effects on fish reproduction remain unknown. In the present study, 4-month-old zebrafish were exposed to different concentrations of 8:2 FTOH (0, 10, 30, 90, 270mug/L) for 4 weeks, and the impact on reproduction was investigated. The plasma testosterone (T) and estradiol (E2) levels were significantly increased in the females, while T and E2 levels were decreased and increased in the males, respectively. The average number of eggs spawned and sperm production were reduced upon exposure to the chemical, which also resulted in thinning of eggshell, and reduced protein content and egg diameter. Histological examination showed promotion of oocyte maturation and retarded spermiation in female and male fish, respectively. The gene transcription of follicle-stimulating hormone beta (FSHbeta) and luteinizing hormone beta (LHbeta) in the pituitary were up- and down-regulated in female and male fish, respectively. Up-regulation of vitellogenin (VTG1) and zona pellucida protein 2 (ZP2a) gene transcription was observed in the males, indicating estrogenic activity. However, down-regulation of gene transcription of VTG1 and ZP2a was measured in the females, associated with decreased fecundity. In addition, exposure of adult zebrafish to 8:2 FTOH caused reduced hatching rates in the offspring. The results demonstrated that waterborne exposure to 8:2 FTOH caused disruption of sex hormone biosynthesis and impaired reproduction in adult zebrafish, ultimately resulting in decreased hatching rates in the offspring.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping